Relationship between intracellular pH, metabolic co-factors and caspase-3 activation in cancer cells during apoptosis.
Biochim Biophys Acta Mol Cell Res
; 1864(3): 604-611, 2017 Mar.
Article
en En
| MEDLINE
| ID: mdl-28063999
A complex cascade of molecular events occurs in apoptotic cells but cell-to-cell variability significantly complicates determination of the order and interconnections between different processes. For better understanding of the mechanisms of programmed cell death, dynamic simultaneous registration of several parameters is required. In this paper we used multiparameter fluorescence microscopy to analyze energy metabolism, intracellular pH and caspase-3 activation in living cancer cells in vitro during staurosporine-induced apoptosis. We performed metabolic imaging of two co-factors, NAD(P)H and FAD, and used the genetically encoded pH-indicator SypHer1 and the FRET-based sensor for caspase-3 activity, mKate2-DEVD-iRFP, to visualize these parameters by confocal fluorescence microscopy and two-photon fluorescence lifetime imaging microscopy. The correlation between energy metabolism, intracellular pH and caspase-3 activation and their dynamic changes were studied in CT26 cancer cells during apoptosis. Induction of apoptosis was accompanied by a switch to oxidative phosphorylation, cytosol acidification and caspase-3 activation. We showed that alterations in cytosolic pH and the activation of oxidative phosphorylation are relatively early events associated with the induction of apoptosis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Apoptosis
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Estaurosporina
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Células Epiteliales
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Caspasa 3
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Antineoplásicos
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta Mol Cell Res
Año:
2017
Tipo del documento:
Article
Pais de publicación:
Países Bajos