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Mammalian Cell Tissue Culture Techniques.
Phelan, Katy; May, Kristin M.
Afiliación
  • Phelan K; Florida Cancer Specialists and Research Institute, Fort Myers, Florida.
  • May KM; Genetic Diagnostic Laboratory, Children's Hospital at Erlanger, Chattanooga, Tennessee.
Curr Protoc Mol Biol ; 117: A.3F.1-A.3F.23, 2017 01 05.
Article en En | MEDLINE | ID: mdl-28060407
Cultured mammalian cells are used extensively in molecular biology studies. A number of special skills are required in order to preserve the structure, function, behavior, and biology of cells in culture. This appendix describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2017 by John Wiley & Sons, Inc.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Técnicas de Cultivo de Tejidos Límite: Animals / Humans Idioma: En Revista: Curr Protoc Mol Biol Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Técnicas de Cultivo de Tejidos Límite: Animals / Humans Idioma: En Revista: Curr Protoc Mol Biol Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos