Biochemical characterization of the DNA polymerase activity present in isolated nuclei from mussel tissues: a possible system for the evaluation of the rate of DNA repair.
Comp Biochem Physiol B
; 93(4): 747-51, 1989.
Article
en En
| MEDLINE
| ID: mdl-2805640
1. The nuclear DNA polymerase activity in mussel digestive glands was characterized regarding Mg++ requirement (2 mM), ATP concentration (4 mM), pH (8.4), and ionic strength (50 mM). 2. Most of the enzymatic activity is aphidicolin insensitive, probably due to DNA polymerase beta-like activity. 3. The exposure of mussels to a genotoxic organic compound such as dimethylsulfate (DMS) resulted in increases of about 250% and 100% in the DNA polymerase activity in nuclei isolated from the gills and digestive glands, respectively. 4. These data indicate that the isolated nuclei from mussel tissues may be utilized for the evaluation of the rate of DNA repair synthesis that reflects the DNA damage induced in vivo by exposure of the animals to genotoxic compounds.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Núcleo Celular
/
ADN Polimerasa Dirigida por ADN
Límite:
Animals
Idioma:
En
Revista:
Comp Biochem Physiol B
Año:
1989
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Reino Unido