Split-BioID: a proximity biotinylation assay for dimerization-dependent protein interactions.

De Munter, Sofie; Görnemann, Janina; Derua, Rita; Lesage, Bart; Qian, Junbin; Heroes, Ewald; Waelkens, Etienne; Van Eynde, Aleyde; Beullens, Monique; Bollen, Mathieu

De Munter, Sofie; Görnemann, Janina; Derua, Rita; Lesage, Bart; Qian, Junbin; Heroes, Ewald; Waelkens, Etienne; Van Eynde, Aleyde; Beullens, Monique; Bollen, Mathieu.

Afiliación

De Munter S; Laboratory of Biosignaling & Therapeutics, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, Belgium.
Görnemann J; Laboratory of Biosignaling & Therapeutics, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, Belgium.
Derua R; Protein Phosphorylation & Proteomics Lab, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, Belgium.
Lesage B; SyBioMa, KU Leuven, Belgium.
Qian J; Laboratory of Biosignaling & Therapeutics, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, Belgium.
Heroes E; Laboratory of Biosignaling & Therapeutics, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, Belgium.
Waelkens E; Laboratory of Biosignaling & Therapeutics, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, Belgium.
Van Eynde A; Protein Phosphorylation & Proteomics Lab, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, Belgium.
Beullens M; SyBioMa, KU Leuven, Belgium.
Bollen M; Laboratory of Biosignaling & Therapeutics, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, Belgium.

FEBS Lett ; 591(2): 415-424, 2017 01.

Article en En | MEDLINE | ID: mdl-28032891

Asunto(s)

Bioensayo/métodos; Dimerización; Mapeo de Interacción de Proteínas; Biotinilación; Prueba de Complementación Genética; Células HEK293; Humanos; Reproducibilidad de los Resultados

Palabras clave

biotinylation; phosphatase-substrate mapping; protein-ligand screening; proximity labeling; reporter-fragment complementation

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bioensayo / Dimerización / Mapeo de Interacción de Proteínas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: FEBS Lett Año: 2017 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido

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