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Changes in acetylation of lysine 5 on histone H4 in canine oocytes following in vitro maturation.
Motheo, T F; Arnold, D R; Padilha-Nakaghi, L C; Pires-Buttler, E A; Alves, A E; Apparicio, M; Vicente, Wrr; Lopes, F L.
Afiliación
  • Motheo TF; Department of Animal Reproduction, Faculdade de Ciencias Agrarias e Veterinarias Campus de Jaboticabal, Universidade Estadual Paulista Julio de Mesquita Filho, Jaboticabal, Brazil.
  • Arnold DR; In vitro Brasil S/A, Mogi Mirim, Brazil.
  • Padilha-Nakaghi LC; Department of Animal Reproduction, Faculdade de Ciencias Agrarias e Veterinarias Campus de Jaboticabal, Universidade Estadual Paulista Julio de Mesquita Filho, Jaboticabal, Brazil.
  • Pires-Buttler EA; Department of Animal Reproduction, Faculdade de Ciencias Agrarias e Veterinarias Campus de Jaboticabal, Universidade Estadual Paulista Julio de Mesquita Filho, Jaboticabal, Brazil.
  • Alves AE; Universidade Federal de Uberlandia - Campus Umuarama, Uberlandia, Brazil.
  • Apparicio M; Programa de Mestrado em Ciência Animal, Universidade de Franca, Franca, Brazil.
  • Vicente W; Department of Animal Reproduction, Faculdade de Ciencias Agrarias e Veterinarias Campus de Jaboticabal, Universidade Estadual Paulista Julio de Mesquita Filho, Jaboticabal, Brazil.
  • Lopes FL; Department of Support, Production and Animal Health, Faculdade de Medicina Veterinária de Araçatuba - FMVA/UNESP, Universidade Estadual Júlio de Mesquita Filho, Araçatuba, Brazil.
Reprod Domest Anim ; 52 Suppl 2: 103-107, 2017 Apr.
Article en En | MEDLINE | ID: mdl-28025854
Post-translational modifications of histones, such as acetylation, are involved in regulating chromatin remodelling and gene expression. Proper in vitro maturation (IVM) of canine oocytes, for many reasons, is up to now inefficient. This study aimed to evaluate the post-translational histone H4 acetylation at lysine 5 (H4K5) in immature and post-IVM canine oocytes. Oocyte nuclear stage was assessed using Hoechst 33342 staining. Acetylation patterns were determined by indirect immunofluorescence staining of immature and post-IVM oocytes, using an antibody against the acetylated lysine 5 residue on histone 4 (H4K5ac). The experiment was repeated four times, with a total of 7-17 oocytes evaluated per stage. Immunofluorescence signal was quantified using the NIHimagej software. Data were expressed as a percentage of the average fluorescence intensity of the specific antibody over the intensity of DNA, as determined by Hoescht staining. H4K5ac displayed a significantly higher acetylated pattern in immature oocytes (0.97 ± 0.08) when compared to post-IVM oocytes at different nuclear stages. There was a decrease in the fluorescence level of the matured oocytes with the progression of meiosis (GVBD: 0.47 ± 0.06 and MI/MII: 0.35 ± 0.04). Similarly to other domestic species, we hypothesized that post-translational modification of histone acetylation takes place during meiosis of in vitro matured canine oocytes. However, it remains to be investigated whether these changes occur during in vivo maturation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Oogénesis / Histonas / Técnicas de Maduración In Vitro de los Oocitos / Meiosis Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Oogénesis / Histonas / Técnicas de Maduración In Vitro de los Oocitos / Meiosis Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania