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NKG2A-Expressing Natural Killer Cells Dominate the Response to Autologous Lymphoblastoid Cells Infected with Epstein-Barr Virus.
Hatton, Olivia; Strauss-Albee, Dara Marie; Zhao, Nancy Q; Haggadone, Mikel D; Pelpola, Judith Shanika; Krams, Sheri M; Martinez, Olivia M; Blish, Catherine A.
Afiliación
  • Hatton O; Department of Molecular Biology, Colorado College, Colorado Springs, CO, USA; Program in Immunology, Stanford University School of Medicine, Stanford, CA, USA.
  • Strauss-Albee DM; Program in Immunology, Stanford University School of Medicine , Stanford, CA , USA.
  • Zhao NQ; Program in Immunology, Stanford University School of Medicine , Stanford, CA , USA.
  • Haggadone MD; Program in Immunology, Stanford University School of Medicine , Stanford, CA , USA.
  • Pelpola JS; Program in Immunology, Stanford University School of Medicine , Stanford, CA , USA.
  • Krams SM; Program in Immunology, Stanford University School of Medicine, Stanford, CA, USA; Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Martinez OM; Program in Immunology, Stanford University School of Medicine, Stanford, CA, USA; Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Blish CA; Program in Immunology, Stanford University School of Medicine, Stanford, CA, USA; Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Front Immunol ; 7: 607, 2016.
Article en En | MEDLINE | ID: mdl-28018364
Epstein-Barr virus (EBV) is a human γ-herpesvirus that establishes latency and lifelong infection in host B cells while achieving a balance with the host immune response. When the immune system is perturbed through immunosuppression or immunodeficiency, however, these latently infected B cells can give rise to aggressive B cell lymphomas. Natural killer (NK) cells are regarded as critical in the early immune response to viral infection, but their role in controlling expansion of infected B cells is not understood. Here, we report that NK cells from healthy human donors display increased killing of autologous B lymphoblastoid cell lines (LCLs) harboring latent EBV compared to primary B cells. Coculture of NK cells with autologous EBV+ LCL identifies an NK cell population that produces IFNγ and mobilizes the cytotoxic granule protein CD107a. Multi-parameter flow cytometry and Boolean analysis reveal that these functional cells are enriched for expression of the NK cell receptor NKG2A. Further, NKG2A+ NK cells more efficiently lyse autologous LCL than do NKG2A- NK cells. More specifically, NKG2A+2B4+CD16-CD57-NKG2C-NKG2D+ cells constitute the predominant NK cell population that responds to latently infected autologous EBV+ B cells. Thus, a subset of NK cells is enhanced for the ability to recognize and eliminate autologous, EBV-infected transformed cells, laying the groundwork for harnessing this subset for therapeutic use in EBV+ malignancies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Immunol Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Immunol Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza