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Several Hfq-dependent alterations in physiology of Yersinia enterocolitica O:3 are mediated by derepression of the transcriptional regulator RovM.
Leskinen, Katarzyna; Pajunen, Maria I; Varjosalo, Markku; Fernández-Carrasco, Helena; Bengoechea, José A; Skurnik, Mikael.
Afiliación
  • Leskinen K; Department of Bacteriology and Immunology, Medicum, Research Programs Unit, Immunobiology, University of Helsinki, Finland.
  • Pajunen MI; Department of Bacteriology and Immunology, Medicum, Research Programs Unit, Immunobiology, University of Helsinki, Finland.
  • Varjosalo M; Institute of Biotechnology, University of Helsinki.
  • Fernández-Carrasco H; Biocentrum Helsinki, Finland: Finnish Institute of Molecular Medicine, Finland.
  • Bengoechea JA; Centre for Experimental Medicine, Queens University Belfast, Belfast, UK.
  • Skurnik M; Centre for Experimental Medicine, Queens University Belfast, Belfast, UK.
Mol Microbiol ; 103(6): 1065-1091, 2017 03.
Article en En | MEDLINE | ID: mdl-28010054
In bacteria, the RNA chaperone Hfq enables pairing of small regulatory RNAs with their target mRNAs and therefore is a key player of post-transcriptional regulation network. As a global regulator, Hfq is engaged in the adaptation to external environment, regulation of metabolism and bacterial virulence. In this study we used RNA-sequencing and quantitative proteomics (LC-MS/MS) to elucidate the role of this chaperone in the physiology and virulence of Yersinia enterocolitica serotype O:3. This global approach revealed the profound impact of Hfq on gene and protein expression. Furthermore, the role of Hfq in the cell morphology, metabolism, cell wall integrity, resistance to external stresses and pathogenicity was evaluated. Importantly, our results revealed that several alterations typical for the hfq-negative phenotype were due to derepression of the transcriptional factor RovM. The overexpression of RovM caused by the loss of Hfq chaperone resulted in extended growth defect, alterations in the lipid A structure, motility and biofilm formation defects, as well as changes in mannitol utilization. Furthermore, in Y. enterocolitica RovM only in the presence of Hfq affected the abundance of RpoS. Finally, the impact of hfq and rovM mutations on the virulence was assessed in the mouse infection model.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factor sigma / Proteínas Bacterianas / Factores de Transcripción / Yersinia enterocolitica / Chaperonas Moleculares / Proteína de Factor 1 del Huésped / Secuencias Reguladoras de Ácido Ribonucleico / ARN Pequeño no Traducido / ARN Largo no Codificante Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Mol Microbiol Asunto de la revista: BIOLOGIA MOLECULAR / MICROBIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factor sigma / Proteínas Bacterianas / Factores de Transcripción / Yersinia enterocolitica / Chaperonas Moleculares / Proteína de Factor 1 del Huésped / Secuencias Reguladoras de Ácido Ribonucleico / ARN Pequeño no Traducido / ARN Largo no Codificante Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Mol Microbiol Asunto de la revista: BIOLOGIA MOLECULAR / MICROBIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Reino Unido