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Polymorphisms in K13, pfcrt, pfmdr1, pfdhfr, and pfdhps in parasites isolated from symptomatic malaria patients in Burkina Faso.
Somé, Anyirékun Fabrice; Sorgho, Hermann; Zongo, Issaka; Bazié, Thomas; Nikiéma, Frédéric; Sawadogo, Amadé; Zongo, Moussa; Compaoré, Yves-Daniel; Ouédraogo, Jean-Bosco.
Afiliación
  • Somé AF; Institut de Recherche en Sciences de la Santé, Direction Régionale de l'Ouest, 399 Avenue de la liberté, 01 BP 545, Bobo-Dioulasso 01, Burkina Faso.
  • Sorgho H; Institut de Recherche en Sciences de la Santé, Unité de Recherche Clinique de Nanoro, BP 218 Ouaga CMS 11, Burkina Faso.
  • Zongo I; Institut de Recherche en Sciences de la Santé, Direction Régionale de l'Ouest, 399 Avenue de la liberté, 01 BP 545, Bobo-Dioulasso 01, Burkina Faso.
  • Bazié T; Institut de Recherche en Sciences de la Santé, Direction Régionale de l'Ouest, 399 Avenue de la liberté, 01 BP 545, Bobo-Dioulasso 01, Burkina Faso.
  • Nikiéma F; Institut de Recherche en Sciences de la Santé, Direction Régionale de l'Ouest, 399 Avenue de la liberté, 01 BP 545, Bobo-Dioulasso 01, Burkina Faso.
  • Sawadogo A; Institut de Recherche en Sciences de la Santé, Direction Régionale de l'Ouest, 399 Avenue de la liberté, 01 BP 545, Bobo-Dioulasso 01, Burkina Faso.
  • Zongo M; Institut de Recherche en Sciences de la Santé, Direction Régionale de l'Ouest, 399 Avenue de la liberté, 01 BP 545, Bobo-Dioulasso 01, Burkina Faso.
  • Compaoré YD; Institut de Recherche en Sciences de la Santé, Direction Régionale de l'Ouest, 399 Avenue de la liberté, 01 BP 545, Bobo-Dioulasso 01, Burkina Faso.
  • Ouédraogo JB; Institut de Recherche en Sciences de la Santé, Direction Régionale de l'Ouest, 399 Avenue de la liberté, 01 BP 545, Bobo-Dioulasso 01, Burkina Faso.
Parasite ; 23: 60, 2016.
Article en En | MEDLINE | ID: mdl-28004634
BACKGROUND: The emergence of resistance to artemisinin derivatives in western Cambodia is threatening to revert the recent advances made toward global malaria control and elimination. Known resistance-mediating polymorphisms in the K13, pfcrt, pfmdr1, pfdhfr, and pfdhps genes are of greatest importance for monitoring the spread of antimalarial drug resistance. METHODS: Samples for the present study were collected from 244 patients with uncomplicated malaria in health centers of Bobo-Dioulasso, Burkina Faso. Blood sample was collected on filter paper before the subject received any treatment. The parasite DNA was then extracted and amplified by Polymerase Chain Reaction (PCR) to evaluate the prevalence of polymorphism of pfcrtK76T, pfmdr1 (N86Y, Y184F), and pfdhps (A437G, K540E). The K13 gene polymorphism was analyzed by nested PCR followed by sequencing. RESULTS: The overall results showed 2.26% (5/221) of K13 synonymous mutant alleles (two C469C, one Y493Y, one G496G, and one V589V), 24.78%, 19.58%, 68.75%, 60.9%, 53.7%, 63.8%, and 64.28%, respectively, for mutant pfcrt 76T, pfmdr1-86Y, pfmdr1-184F, pfdhfr51I, pfdhfr59R, pfdhfr108N, and pfdhps 437G. We did not report any mutation at codon 540 of pfdhps. CONCLUSION: These results provide baseline prevalence of known drug resistance polymorphisms and suggest that artemisinin combination therapies may retain good efficacy in the treatment of uncomplicated malaria in Burkina Faso.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium falciparum / Proteínas Protozoarias / Malaria Falciparum / Polimorfismo de Nucleótido Simple Tipo de estudio: Diagnostic_studies / Risk_factors_studies Límite: Adolescent / Adult / Child / Child, preschool / Humans / Infant País/Región como asunto: Africa Idioma: En Revista: Parasite Asunto de la revista: PARASITOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Burquina Faso Pais de publicación: Francia
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium falciparum / Proteínas Protozoarias / Malaria Falciparum / Polimorfismo de Nucleótido Simple Tipo de estudio: Diagnostic_studies / Risk_factors_studies Límite: Adolescent / Adult / Child / Child, preschool / Humans / Infant País/Región como asunto: Africa Idioma: En Revista: Parasite Asunto de la revista: PARASITOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Burquina Faso Pais de publicación: Francia