Induction of protective immunity against influenza A/Jiangxi-Donghu/346/2013 (H10N8) in mice.
J Gen Virol
; 98(2): 155-165, 2017 02.
Article
en En
| MEDLINE
| ID: mdl-27983474
Human infections with A/Jiangxi-Donghu/346/2013 (H10N8) virus have raised concerns about its pandemic potential. In order to develop a vaccine against this virus, the immunogenicity of its haemagglutinin protein was evaluated in mice. Using both whole-virion and recombinant subunit protein vaccines, we showed that two doses of either vaccine elicited neutralizing antibody responses. The protective efficacy of the vaccine-induced responses was assessed using a reverse-genetics-derived H10 reassortant virus on the A/Puerto Rico/8/34 (H1N1) backbone. The reassortant virus replicated efficiently in the respiratory tract of unvaccinated mice whereas vaccinated mice were completely protected from challenge, with no detectable viral load in the lower respiratory tract. Finally, the serum neutralizing antibody responses elicited by the H10 vaccines also exhibited cross-neutralizing activity against three heterologous wild-type H10 viruses. Collectively, these findings demonstrate that different vaccine platforms presenting the H10 haemagglutinin protein induce protective immunity.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vacunas contra la Influenza
/
Infecciones por Orthomyxoviridae
/
Glicoproteínas Hemaglutininas del Virus de la Influenza
/
Anticuerpos Neutralizantes
/
Subtipo H10N8 del Virus de la Influenza A
/
Inmunogenicidad Vacunal
/
Anticuerpos Antivirales
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Gen Virol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Singapur
Pais de publicación:
Reino Unido