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Site-Selective Surface-Enhanced Raman Detection of Proteins.
Matteini, Paolo; Cottat, Maximilien; Tavanti, Francesco; Panfilova, Elizaveta; Scuderi, Mario; Nicotra, Giuseppe; Menziani, Maria Cristina; Khlebtsov, Nikolai; de Angelis, Marella; Pini, Roberto.
Afiliación
  • Matteini P; Institute of Applied Physics "Nello Carrara", National Research Council , via Madonna del Piano 10, 50019 Sesto Fiorentino, Italy.
  • Cottat M; Institute of Applied Physics "Nello Carrara", National Research Council , via Madonna del Piano 10, 50019 Sesto Fiorentino, Italy.
  • Tavanti F; Department of Chemical and Geological Sciences, University of Modena e Reggio Emilia , via Campi 103, 41125 Modena, Italy.
  • Panfilova E; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences , 13 Prospekt Entuziastov, 410049 Saratov, Russia.
  • Scuderi M; Institute for Microelectronics and Microsystems, National Research Council , zona industriale strada VIII n.5, 95121 Catania, Italy.
  • Nicotra G; Institute for Microelectronics and Microsystems, National Research Council , zona industriale strada VIII n.5, 95121 Catania, Italy.
  • Menziani MC; Department of Chemical and Geological Sciences, University of Modena e Reggio Emilia , via Campi 103, 41125 Modena, Italy.
  • Khlebtsov N; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences , 13 Prospekt Entuziastov, 410049 Saratov, Russia.
  • de Angelis M; Saratov National Research State University , 83 Ulitsa Astrakhanskaya, 410012 Saratov, Russia.
  • Pini R; Institute of Applied Physics "Nello Carrara", National Research Council , via Madonna del Piano 10, 50019 Sesto Fiorentino, Italy.
ACS Nano ; 11(1): 918-926, 2017 01 24.
Article en En | MEDLINE | ID: mdl-27960057
Strategies for protein detection via surface-enhanced Raman spectroscopy (SERS) currently exploit the formation of randomly generated hot spots at the interfaces of metal colloidal nanoparticles, which are clustered together by intrusive chemical or physical processes in the presence of the target biomolecule. We propose a different approach based on selective and quantitative gathering of protein molecules at regular hot spots generated on the corners of individual silver nanocubes in aqueous medium at physiological pH. Here, the protein, while keeping its native configuration, experiences an intense local E-field, which boosts SERS efficiency and detection sensitivity. Uncontrolled signal fluctuations caused by variable molecular adsorption to different particle areas or inside clustered nanoparticles are circumvented. Advanced electron microscopy analyses and computational simulations outline a strategy relying on a site-selective mechanism with superior Raman signal enhancement, which offers the perspective of highly controlled and reproducible routine SERS detection of proteins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría Raman / Proteínas Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: ACS Nano Año: 2017 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría Raman / Proteínas Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: ACS Nano Año: 2017 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos