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Responses of primary cultured haemocytes derived from the marine gastropod Haliotis tuberculata to an industrial effluent exposure.
Ladhar-Chaabouni, Rim; Houel, Thomas; Serpentini, Antoine; Karray, Sahar; Lebel, Jean-Marc; Hamza-Chaffai, Amel.
Afiliación
  • Ladhar-Chaabouni R; Marine Ecotoxicology UR 09-03, IPEIS, BP 805, 3018, Sfax, Tunisia. rladhar@yahoo.fr.
  • Houel T; Normandie université, 14032, Caen, France.
  • Serpentini A; UMR BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, 14032, Caen Cedex 5, France.
  • Karray S; Normandie université, 14032, Caen, France.
  • Lebel JM; UMR BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, 14032, Caen Cedex 5, France.
  • Hamza-Chaffai A; Marine Ecotoxicology UR 09-03, IPEIS, BP 805, 3018, Sfax, Tunisia.
Cytotechnology ; 69(2): 191-200, 2017 Apr.
Article en En | MEDLINE | ID: mdl-27957649
This study assessed the responses of primary cultured haemocytes from the marine gastropod Haliotis tuberculata exposed to the increasing concentrations of industrial effluent (0, 0.5, 1, 10, 15 and 20%) discharged into the Tunisian coastal area. Analyses showed the presence of metals such as cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), zinc (Zn), nickel (Ni) and lead (Pb) in the effluent. The effects of this mixture of pollutants on abalone haemocyte parameters were reflected by a significant decrease of cell viability, phagocytotic activity and reactive oxygen species (ROS) production as well as morphological and lysosomal membrane alterations. Thus, these results indicated that our primary culture system represents a suitable in vitro model for monitoring of anthropogenic contaminants in aquatic environments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Cytotechnology Asunto de la revista: BIOTECNOLOGIA / GENETICA Año: 2017 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Cytotechnology Asunto de la revista: BIOTECNOLOGIA / GENETICA Año: 2017 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Estados Unidos