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Osteoblast adhesion, migration, and proliferation variations on chemically patterned nanocrystalline diamond films evaluated by live-cell imaging.
Broz, Antonin; Ukraintsev, Egor; Kromka, Alexander; Rezek, Bohuslav; Hubalek Kalbacova, Marie.
Afiliación
  • Broz A; Institute of Inherited Metabolic Disorders, Laboratory of Interaction of Cells with Nanomaterials, 1st Faculty of Medicine, Charles University in Prague, Ke Karlovu 2, 12853 Prague 2, Czech Republic.
  • Ukraintsev E; Institute of Physiology, Department of Biomaterials and Tissue Engineering, Czech Academy of Sciences, v. v. i, Videnska 1083, 142 20, Prague 4, Czech Republic.
  • Kromka A; Institute of Physics, Department of Optical Materials, Czech Academy of Sciences, v. v. i, Cukrovarnicka 10, 162 53 Prague 6, Czech Republic.
  • Rezek B; Institute of Physics, Department of Optical Materials, Czech Academy of Sciences, v. v. i, Cukrovarnicka 10, 162 53 Prague 6, Czech Republic.
  • Hubalek Kalbacova M; Institute of Physics, Department of Optical Materials, Czech Academy of Sciences, v. v. i, Cukrovarnicka 10, 162 53 Prague 6, Czech Republic.
J Biomed Mater Res A ; 105(5): 1469-1478, 2017 05.
Article en En | MEDLINE | ID: mdl-27935192
Cell fate modulation by adapting the surface of a biocompatible material is nowadays a challenge in implantology, tissue engineering as well as in construction of biosensors. Nanocrystalline diamond (NCD) thin films are considered promising in these fields due to their extraordinary physical and chemical properties and diverse ways in which they can be modified structurally and chemically. The initial cell distribution, the rate of cell adhesion, distance of cell migration and also the cell proliferation are influenced by the NCD surface termination. Here, we use real-time live-cell imaging to investigate the above-mentioned processes on oxidized NCD (NCD-O) and hydrogenated NCD (NCD-H) to elucidate cell preference to the NCD-O especially on surfaces with microscopic surface termination patterns. Cells adhere more slowly and migrate farther on NCD-H than on NCD-O. Cells seeded with a fetal bovine serum (FBS) supplement in the medium move across the surface prior to adhesion. In the absence of FBS, the cells adhere immediately, but still exhibit different migration and proliferation on NCD-O/H regions. We discuss the impact of these effects on the formation of cell arrays on micropatterned NCD. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1469-1478, 2017.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Movimiento Celular / Proliferación Celular / Nanodiamantes / Membranas Artificiales Límite: Humans Idioma: En Revista: J Biomed Mater Res A Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2017 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Movimiento Celular / Proliferación Celular / Nanodiamantes / Membranas Artificiales Límite: Humans Idioma: En Revista: J Biomed Mater Res A Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2017 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Estados Unidos