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Results of rat Pig-a/PIGRET assay with a single dose regimen of 1,3-propane sultone and 2-acetyl aminofluorene.
Shigano, Miyuki; Ishii, Nana; Takashima, Rie; Harada, Hideki; Takasawa, Hironao; Hamada, Shuichi.
Afiliación
  • Shigano M; LSI Medience Corporation, 14-1 Sunayama, Kamisu-shi, Ibaraki 314-0255, Japan. Electronic address: shigano.miyuki@ma.medience.co.jp.
  • Ishii N; LSI Medience Corporation, 14-1 Sunayama, Kamisu-shi, Ibaraki 314-0255, Japan.
  • Takashima R; Tsukubamirai-shi, Ibaraki 300-2358, Japan.
  • Harada H; LSI Medience Corporation, 14-1 Sunayama, Kamisu-shi, Ibaraki 314-0255, Japan.
  • Takasawa H; LSI Medience Corporation, 14-1 Sunayama, Kamisu-shi, Ibaraki 314-0255, Japan.
  • Hamada S; LSI Medience Corporation, 14-1 Sunayama, Kamisu-shi, Ibaraki 314-0255, Japan.
Mutat Res Genet Toxicol Environ Mutagen ; 811: 75-79, 2016 Nov 15.
Article en En | MEDLINE | ID: mdl-27931819
The Pig-a assay is a useful in vivo mutation detecting test and is easier to perform than the in vivo transgenic mutation assay. This assay is now recognized to be able to detect a number of mutagenic chemicals administered to rats in sub-acute or sub-chronic dose studies. The present investigation was conducted to evaluate the usefulness of peripheral blood Pig-a assays with total red blood cells (RBC Pig-a assay) and with reticulocytes (PIGRET assay) using two genotoxic rodent carcinogens, 1,3-propane sultone (1,3-PS) and 2-acetylaminofluorene (2-AAF). Male rats were orally administered a single dose of each test compound, and both the RBC Pig-a and PIGRET assays were performed using flow cytometry to measure the Pig-a mutant frequency (MF) before and after dosing on Days 8, 15 and 29. In the experiment with 1,3-PS, significant increases in Pig-a MF were observed from Day 15 and Day 8 in the RBC Pig-a and PIGRET assays, respectively. The results of both assays demonstrated that the increases in Pig-a MF were detectable after a single treatment with 1,3-PS. Furthermore, the difference in the kinetics of the increase in Pig-a MF between the RBC Pig-a and PIGRET assays with 1,3-PS suggests that the PIGRET assay has an advantage in detecting the mutant erythrocytes earlier than the RBC Pig-a assay. In contrast, no significant increases were observed in the Pig-a assays using either RBC or reticulocytes with 2-AAF. The negative results in both assays with 2-AAF may indicate the limitation of the single dose method; however, further investigation at higher doses is necessary to determine limitation of the single dose method.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: 2-Acetilaminofluoreno / Reticulocitos / Tiofenos / Eritrocitos / Proteínas de la Membrana / Pruebas de Mutagenicidad / Mutágenos Límite: Animals Idioma: En Revista: Mutat Res Genet Toxicol Environ Mutagen Año: 2016 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: 2-Acetilaminofluoreno / Reticulocitos / Tiofenos / Eritrocitos / Proteínas de la Membrana / Pruebas de Mutagenicidad / Mutágenos Límite: Animals Idioma: En Revista: Mutat Res Genet Toxicol Environ Mutagen Año: 2016 Tipo del documento: Article Pais de publicación: Países Bajos