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Effect of TREM-1 blockade and single nucleotide variants in experimental renal injury and kidney transplantation.
Tammaro, Alessandra; Kers, Jesper; Emal, Diba; Stroo, Ingrid; Teske, Gwendoline J D; Butter, Loes M; Claessen, Nike; Damman, Jeffrey; Derive, Marc; Navis, Gerjan J; Florquin, Sandrine; Leemans, Jaklien C; Dessing, Mark C.
Afiliación
  • Tammaro A; Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
  • Kers J; Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
  • Emal D; Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
  • Stroo I; Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
  • Teske GJD; Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
  • Butter LM; Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
  • Claessen N; Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
  • Damman J; Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
  • Derive M; Inserm UMR_S1116, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-les-Nancy, France.
  • Navis GJ; Department of Internal Medicine, Division of Nephrology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
  • Florquin S; Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
  • Leemans JC; Department of Pathology, Radboud University Nijmegen Medical Center, Nijmegen, the Netherlands.
  • Dessing MC; Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
Sci Rep ; 6: 38275, 2016 12 08.
Article en En | MEDLINE | ID: mdl-27928159
Renal ischemia reperfusion (IR)-injury induces activation of innate immune response which sustains renal injury and contributes to the development of delayed graft function (DGF). Triggering receptor expressed on myeloid cells-1 (TREM-1) is a pro-inflammatory evolutionary conserved pattern recognition receptor expressed on a variety of innate immune cells. TREM-1 expression increases following acute and chronic renal injury. However, the function of TREM-1 in renal IR is still unclear. Here, we investigated expression and function of TREM-1 in a murine model of renal IR using different TREM-1 inhibitors: LP17, LR12 and TREM-1 fusion protein. In a human study, we analyzed the association of non-synonymous single nucleotide variants in the TREM1 gene in a cohort comprising 1263 matching donors and recipients with post-transplant outcomes, including DGF. Our findings demonstrated that, following murine IR, renal TREM-1 expression increased due to the influx of Trem1 mRNA expressing cells detected by in situ hybridization. However, TREM-1 interventions by means of LP17, LR12 and TREM-1 fusion protein did not ameliorate IR-induced injury. In the human renal transplant cohort, donor and recipient TREM1 gene variant p.Thr25Ser was not associated with DGF, nor with biopsy-proven rejection or death-censored graft failure. We conclude that TREM-1 does not play a major role during experimental renal IR and after kidney transplantation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión / Funcionamiento Retardado del Injerto / Receptor Activador Expresado en Células Mieloides 1 / Inflamación Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión / Funcionamiento Retardado del Injerto / Receptor Activador Expresado en Células Mieloides 1 / Inflamación Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido