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Label-Free Virus Capture and Release by a Microfluidic Device Integrated with Porous Silicon Nanowire Forest.
Xia, Yiqiu; Tang, Yi; Yu, Xu; Wan, Yuan; Chen, Yizhu; Lu, Huaguang; Zheng, Si-Yang.
Afiliación
  • Xia Y; Department of Biomedical Engineering, Micro and Nano Integrated Biosystem (MINIBio) Laboratory, The Pennsylvania State University, University Park, PA, 16802, USA.
  • Tang Y; Department of Veterinary and Biomedical Science, The Pennsylvania State University, University Park, PA, 16802, USA.
  • Yu X; Department of Biomedical Engineering, Micro and Nano Integrated Biosystem (MINIBio) Laboratory, The Pennsylvania State University, University Park, PA, 16802, USA.
  • Wan Y; Department of Biomedical Engineering, Micro and Nano Integrated Biosystem (MINIBio) Laboratory, The Pennsylvania State University, University Park, PA, 16802, USA.
  • Chen Y; Department of Electrical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.
  • Lu H; Department of Veterinary and Biomedical Science, The Pennsylvania State University, University Park, PA, 16802, USA.
  • Zheng SY; Department of Biomedical Engineering, Micro and Nano Integrated Biosystem (MINIBio) Laboratory, The Pennsylvania State University, University Park, PA, 16802, USA.
Small ; 13(6)2017 Feb.
Article en En | MEDLINE | ID: mdl-27918640
Viral diseases are perpetual threats to human and animal health. Detection and characterization of viral pathogens require accurate, sensitive, and rapid diagnostic assays. For field and clinical samples, the sample preparation procedures limit the ultimate performance and utility of the overall virus diagnostic protocols. This study presents the development of a microfluidic device embedded with porous silicon nanowire (pSiNW) forest for label-free size-based point-of-care virus capture in a continuous curved flow design. The pSiNW forests with specific interwire spacing are synthesized in situ on both bottom and sidewalls of the microchannels in a batch process. With the enhancement effect of Dean flow, this study demonstrates that about 50% H5N2 avian influenza viruses are physically trapped without device clogging. A unique feature of the device is that captured viruses can be released by inducing self-degradation of the pSiNWs in physiological aqueous environment. About 60% of captured viruses can be released within 24 h for virus culture, subsequent molecular diagnosis, and other virus characterization and analyses. This device performs viable, unbiased, and label-free virus isolation and release. It has great potentials for virus discovery, virus isolation and culture, functional studies of virus pathogenicity, transmission, drug screening, and vaccine development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Influenza A / Silicio / Coloración y Etiquetado / Técnicas Analíticas Microfluídicas / Nanocables Tipo de estudio: Guideline Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Influenza A / Silicio / Coloración y Etiquetado / Técnicas Analíticas Microfluídicas / Nanocables Tipo de estudio: Guideline Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania