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Subtilosin Prevents Biofilm Formation by Inhibiting Bacterial Quorum Sensing.
Algburi, Ammar; Zehm, Saskia; Netrebov, Victoria; Bren, Anzhelica B; Chistyakov, Vladimir; Chikindas, Michael L.
Afiliación
  • Algburi A; Department of Biochemistry and Microbiology, Rutgers State University, New Brunswick, NJ, 08901, USA.
  • Zehm S; Department of Microbiology, Veterinary College, Diyala University, Baqubah, Iraq.
  • Netrebov V; Department of Life Sciences and Technology, Beuth University of Applied Sciences, Berlin, Germany.
  • Bren AB; Health Promoting Naturals Laboratory, School of Environmental and Biological Sciences, Rutgers State University, New Brunswick, NJ, USA.
  • Chistyakov V; D. I. Ivanovsky Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, Russia.
  • Chikindas ML; D. I. Ivanovsky Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, Russia.
Probiotics Antimicrob Proteins ; 9(1): 81-90, 2017 03.
Article en En | MEDLINE | ID: mdl-27914001
Subtilosin, the cyclic lantibiotic protein produced by Bacillus subtilis KATMIRA1933, targets the surface receptor and electrostatically binds to the bacterial cell membrane. In this study, subtilosin was purified using ammonium sulfate ((NH4)2SO4) precipitation and purified via column chromatography. Subtilosin's antibacterial minimum and sub-minimum inhibitory concentrations (MIC and sub-MIC) and anti-biofilm activity (biofilm prevention) were established. Subtilosin was evaluated as a quorum sensing (QS) inhibitor in Gram-positive bacteria using Fe(III) reduction assay. In Gram-negative bacteria, subtilosin was evaluated as a QS inhibitor utilizing Chromobacterium voilaceum as a microbial reporter. The results showed that Gardnerella vaginalis was more sensitive to subtilosin with MIC of 6.25 µg/mL when compared to Listeria monocytogenes (125 µg/mL). The lowest concentration of subtilosin, at which more than 90% of G. vaginalis biofilm was inhibited without effecting the growth of planktonic cells, was 0.78 µg/mL. About 80% of L. monocytogenes and more than 60% of Escherichia coli biofilm was inhibited when 15.1 µg/mL of subtilosin was applied. Subtilosin with 7.8-125 µg/mL showed a significant reduction in violacein production without any inhibitory effect on the growth of C. violaceum. Subtilosin at 3 and 4 µg/mL reduced the level of Autoinducer-2 (AI-2) production in G. vaginalis. However, subtilosin did not influence AI-2 production by L. monocytogenes at sub-MICs of 0.95-15.1 µg/mL. To our knowledge, this is the first report exploring the relationship between biofilm prevention and quorum sensing inhibition in G. vaginalis using subtilosin as a quorum sensing inhibitor.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos Cíclicos / Bacteriocinas / Biopelículas / Percepción de Quorum / Bacterias Gramnegativas / Bacterias Grampositivas / Antibacterianos Idioma: En Revista: Probiotics Antimicrob Proteins Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos Cíclicos / Bacteriocinas / Biopelículas / Percepción de Quorum / Bacterias Gramnegativas / Bacterias Grampositivas / Antibacterianos Idioma: En Revista: Probiotics Antimicrob Proteins Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos