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Birth of Cloned Microminipigs Derived from Somatic Cell Nuclear Transfer Embryos That Have Been Transiently Treated with Valproic Acid.
Miyoshi, Kazuchika; Kawaguchi, Hiroaki; Maeda, Kosuke; Sato, Masahiro; Akioka, Kohei; Noguchi, Michiko; Horiuchi, Masahisa; Tanimoto, Akihide.
Afiliación
  • Miyoshi K; 1 Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University , Kagoshima, Japan .
  • Kawaguchi H; 2 Department of Hygiene and Health Promotion Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University , Kagoshima, Japan .
  • Maeda K; 1 Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University , Kagoshima, Japan .
  • Sato M; 3 Section of Gene Expression Regulation, Center for Advanced Biomedical Science and Swine Research, Kagoshima University , Kagoshima, Japan .
  • Akioka K; 4 Department of Veterinary Histopathology, Joint Faculty of Veterinary Medicine, Kagoshima University , Kagoshima, Japan .
  • Noguchi M; 5 Laboratory of Theriogenology, Faculty of Veterinary Medicine, Azabu University , Kanagawa, Japan .
  • Horiuchi M; 2 Department of Hygiene and Health Promotion Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University , Kagoshima, Japan .
  • Tanimoto A; 6 Department of Pathology, Graduate School of Medical and Dental Sciences, Kagoshima University , Kagoshima, Japan .
Cell Reprogram ; 18(6): 390-400, 2016 11.
Article en En | MEDLINE | ID: mdl-27906585
In our previous study, we found that treatment of miniature pig somatic cell nuclear transfer (SCNT) embryos with 4 mM valproic acid (VPA), a histone deacetylase inhibitor, for 48 hours after activation enhanced blastocyst formation rate and octamer-binding transcription factor-3/4 (Oct-3/4) gene expression at the late blastocyst stage; however, the production of viable cloned pups failed, when those VPA-treated SCNT embryos were transferred to recipients. This failure suggests that the present VPA treatment is suboptimal. In the present study, we explored the optimal conditions for VPA to have beneficial effects on the development of SCNT embryos. When miniature pig SCNT embryos were treated with 8 mM VPA for 24 hours after activation, both the rates of blastocyst formation and blastocysts expressing the Oct-3/4 gene were significantly (p < 0.05) improved. A similar increase in blastocyst formation was also observed when microminipig-derived cells were used as SCNT donors. Five cloned piglets were obtained after the transfer of 152 microminipig SCNT embryos that had been treated with 8 mM VPA for 24 hours. The results indicated that a short duration of treatment with VPA improves the development of both miniature pig and microminipig SCNT embryos, possibly via an enhanced reprogramming mechanism.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Blastocisto / Ácido Valproico / Clonación de Organismos / Desarrollo Embrionario / Embrión de Mamíferos / Transferencia de Embrión / Técnicas de Transferencia Nuclear Límite: Animals Idioma: En Revista: Cell Reprogram Año: 2016 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Buscar en Google
Añadir a Mi BVS
Imprimir
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Blastocisto / Ácido Valproico / Clonación de Organismos / Desarrollo Embrionario / Embrión de Mamíferos / Transferencia de Embrión / Técnicas de Transferencia Nuclear Límite: Animals Idioma: En Revista: Cell Reprogram Año: 2016 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos