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Carbohydrate metabolism in Oenococcus oeni: a genomic insight.
Cibrario, Alice; Peanne, Claire; Lailheugue, Marine; Campbell-Sills, Hugo; Dols-Lafargue, Marguerite.
Afiliación
  • Cibrario A; University of Bordeaux, ISVV, EA 4577, Oenologie, F-33140, Villenave d'Ornon, France.
  • Peanne C; University of Bordeaux, ISVV, EA 4577, Oenologie, F-33140, Villenave d'Ornon, France.
  • Lailheugue M; Bordeaux INP, ISVV, EA 4577, Oenologie, F-33140, Villenave d'Ornon, France.
  • Campbell-Sills H; University of Bordeaux, ISVV, EA 4577, Oenologie, F-33140, Villenave d'Ornon, France.
  • Dols-Lafargue M; University of Bordeaux, ISVV, EA 4577, Oenologie, F-33140, Villenave d'Ornon, France. dols@enscbp.fr.
BMC Genomics ; 17(1): 984, 2016 12 01.
Article en En | MEDLINE | ID: mdl-27905883
BACKGROUND: Oenococcus oeni is the bacterial species that drives malolactic fermentation in most wines. Several studies have described a high intraspecific diversity regarding carbohydrate degradation abilities but the link between the phenotypes and the genes and metabolic pathways has been poorly described. RESULTS: A collection of 41 strains whose genomic sequences were available and representative of the species genomic diversity was analyzed for growth on 18 carbohydrates relevant in wine. The most frequently used substrates (more than 75% of the strains) were glucose, trehalose, ribose, cellobiose, mannose and melibiose. Fructose and L-arabinose were used by about half the strains studied, sucrose, maltose, xylose, galactose and raffinose were used by less than 25% of the strains and lactose, L-sorbose, L-rhamnose, sorbitol and mannitol were not used by any of the studied strains. To identify genes and pathways associated with carbohydrate catabolic abilities, gene-trait matching and a careful analysis of gene mutations and putative complementation phenomena were performed. CONCLUSIONS: For most consumed sugars, we were able to propose putatively associated metabolic pathways. Most associated genes belong to the core genome. O. oeni appears as a highly specialized species, ideally suited to fermented fruit juice and more specifically to wine for a subgroup of strains.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genoma Bacteriano / Metabolismo de los Hidratos de Carbono / Oenococcus Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2016 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genoma Bacteriano / Metabolismo de los Hidratos de Carbono / Oenococcus Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2016 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido