Sequential lignin depolymerization by combination of biocatalytic and formic acid/formate treatment steps.
Appl Microbiol Biotechnol
; 101(6): 2575-2588, 2017 Mar.
Article
en En
| MEDLINE
| ID: mdl-27904924
Lignin, a complex three-dimensional amorphous polymer, is considered to be a potential natural renewable resource for the production of low-molecular-weight aromatic compounds. In the present study, a novel sequential lignin treatment method consisting of a biocatalytic oxidation step followed by a formic acid-induced lignin depolymerization step was developed and optimized using response surface methodology. The biocatalytic step employed a laccase mediator system using the redox mediator 1-hydroxybenzotriazole. Laccases were immobilized on superparamagnetic nanoparticles using a sorption-assisted surface conjugation method allowing easy separation and reuse of the biocatalysts after treatment. Under optimized conditions, as much as 45 wt% of lignin could be solubilized either in aqueous solution after the first treatment or in ethyl acetate after the second (chemical) treatment. The solubilized products were found to be mainly low-molecular-weight aromatic monomers and oligomers. The process might be used for the production of low-molecular-weight soluble aromatic products that can be purified and/or upgraded applying further downstream processes.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Fúngicas
/
Lacasa
/
Enzimas Inmovilizadas
/
Formiatos
/
Lignina
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
Alemania