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Isolation and Characterisation of Bacteriocinand Aggregation-Promoting Factor Production inLactococcus lactis ssp. lactis BGBM50 Strain.
Mirkovic, Nemanja; Radulovic, Zorica; Uzelac, Gordana; Lozo, Jelena; Obradovic, Dragojlo; Topisirovic, Ljubisa; Kojic, Milan.
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  • Mirkovic N; Faculty of Agriculture, University of Belgrade, RS-11080 Belgrade, Serbia; Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,RS-11010 Belgrade, Serbia.
  • Radulovic Z; Faculty of Agriculture, University of Belgrade, RS-11080 Belgrade, Serbia.
  • Uzelac G; Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,RS-11010 Belgrade, Serbia.
  • Lozo J; Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,RS-11010 Belgrade, Serbia; Faculty of Biology, University of Belgrade, RS-11000 Belgrade, Serbia.
  • Obradovic D; Faculty of Agriculture, University of Belgrade, RS-11080 Belgrade, Serbia.
  • Topisirovic L; Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,RS-11010 Belgrade, Serbia.
  • Kojic M; Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,RS-11010 Belgrade, Serbia.
Food Technol Biotechnol ; 53(2): 237-242, 2015 Jun.
Article en En | MEDLINE | ID: mdl-27904354
Lactococcus lactis ssp. lactis BGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Zanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of different sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specific for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Food Technol Biotechnol Año: 2015 Tipo del documento: Article Pais de publicación: Croacia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Food Technol Biotechnol Año: 2015 Tipo del documento: Article Pais de publicación: Croacia