Your browser doesn't support javascript.
loading
Microvirga ossetica sp. nov., a species of rhizobia isolated from root nodules of the legume species Vicia alpestris Steven.
Safronova, Vera I; Kuznetsova, Irina G; Sazanova, Anna L; Belimov, Andrey A; Andronov, Evgeny E; Chirak, Elizaveta R; Osledkin, Yuri S; Onishchuk, Olga P; Kurchak, Oksana N; Shaposhnikov, Alexander I; Willems, Anne; Tikhonovich, Igor A.
Afiliación
  • Safronova VI; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
  • Kuznetsova IG; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
  • Sazanova AL; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
  • Belimov AA; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
  • Andronov EE; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
  • Chirak ER; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
  • Osledkin YS; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
  • Onishchuk OP; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
  • Kurchak ON; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
  • Shaposhnikov AI; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
  • Willems A; Department of Biochemistry & Microbiology, Faculty of Sciences, Ghent University, 9000 Ghent, K.L. Ledeganckstraat 35, Belgium.
  • Tikhonovich IA; Department of Genetics and Biotechnology, Saint Petersburg State University, 199034, St.-Petersburg, Universitetskaya Emb.7/9, Russian Federation.
Int J Syst Evol Microbiol ; 67(1): 94-100, 2017 Jan.
Article en En | MEDLINE | ID: mdl-27902217
Gram-stain-negative strains V5/3MT, V5/5K, V5/5M and V5/13 were isolated from root nodules of Vicia alpestris plants growing in the North Ossetia region (Caucasus). Sequencing of the partial 16S rRNA gene (rrs) and four housekeeping genes (dnaK, gyrB, recA and rpoB) showed that the isolates from V. alpestris were most closely related to the species Microvirga zambiensis (order Rhizobiales, family Methylobacteriaceae) which was described for the single isolate from root nodule of Listia angolensis growing in Zambia. Sequence similarities between the Microvirga-related isolates and M. zambiensis WSM3693T ranged from 98.5 to 98.7 % for rrs and from 79.7 to 95.8 % for housekeeping genes. Cellular fatty acids of the isolates V5/3MT, V5/5K, V5/5M and V5/13 included important amounts of C18 : 1ω7c (54.0-67.2 %), C16 : 0 (6.0-7.8 %), C19 : 0 cyclo ω8c (3.1-10.2 %), summed feature 2 (comprising one or more of iso-C16 : 1 I, C14 : 0 3-OH and unknown ECL 10.938, 5.8-22.5 %) and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 02-OH, 2.9-4.0 %). DNA-DNA hybridization between the isolate V5/3MT and M. zambiensis WSM3693T revealed DNA-DNA relatedness of 35.3 %. Analysis of morphological and physiological features of the novel isolates demonstrated their unique phenotypic profile in comparison with reference strains from closely related species of the genus Microvirga. On the basis of genotypic and phenotypic analysis, a novel species named Microvirga ossetica sp. nov. is proposed. The type strain is V5/3MT (=LMG 29787T=RCAM 02728T). Three additional strains of the species are V5/5K, V5/5M and V5/13.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Vicia / Methylobacteriaceae / Nódulos de las Raíces de las Plantas País/Región como asunto: Asia / Europa Idioma: En Revista: Int J Syst Evol Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Vicia / Methylobacteriaceae / Nódulos de las Raíces de las Plantas País/Región como asunto: Asia / Europa Idioma: En Revista: Int J Syst Evol Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido