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A GC-MS method for the detection and quantitation of ten major drugs of abuse in human hair samples.
Orfanidis, A; Mastrogianni, O; Koukou, A; Psarros, G; Gika, H; Theodoridis, G; Raikos, N.
Afiliación
  • Orfanidis A; Laboratory of Forensic Medicine & Toxicology, Medical School, Aristotle University Thessaloniki, 54124, Greece.
  • Mastrogianni O; Laboratory of Forensic Service of Ministry of Justice of Thessaloniki, Greece.
  • Koukou A; School of Chemistry, Laboratory of Analytical Chemistry, Aristotle University Thessaloniki, 54124, Greece.
  • Psarros G; School of Chemistry, Laboratory of Analytical Chemistry, Aristotle University Thessaloniki, 54124, Greece.
  • Gika H; Laboratory of Forensic Medicine & Toxicology, Medical School, Aristotle University Thessaloniki, 54124, Greece.
  • Theodoridis G; School of Chemistry, Laboratory of Analytical Chemistry, Aristotle University Thessaloniki, 54124, Greece.
  • Raikos N; Laboratory of Forensic Medicine & Toxicology, Medical School, Aristotle University Thessaloniki, 54124, Greece. Electronic address: raikos@med.auth.gr.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1047: 141-150, 2017 Mar 15.
Article en En | MEDLINE | ID: mdl-27884570
A sensitive analytical method has been developed in order to identify and quantify major drugs of abuse (DOA), namely morphine, codeine, 6-monoacetylmorphine, cocaine, ecgonine methyl ester, benzoylecgonine, amphetamine, methamphetamine, methylenedioxymethamphetamine and methylenedioxyamphetamine in human hair. Samples of hair were extracted with methanol under ultrasonication at 50°C after a three step rinsing process to remove external contamination and dirt hair. Derivatization with BSTFA was selected in order to increase detection sensitivity of GC/MS analysis. Optimization of derivatization parameters was based on experiments for the selection of derivatization time, temperature and volume of derivatising agent. Validation of the method included evaluation of linearity which ranged from 2 to 350ng/mg of hair mean concentration for all DOA, evaluation of sensitivity, accuracy, precision and repeatability. Limits of detection ranged from 0.05 to 0.46ng/mg of hair. The developed method was applied for the analysis of hair samples obtained from three human subjects and were found positive in cocaine, and opiates.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Drogas Ilícitas / Detección de Abuso de Sustancias / Cabello / Cromatografía de Gases y Espectrometría de Masas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2017 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Drogas Ilícitas / Detección de Abuso de Sustancias / Cabello / Cromatografía de Gases y Espectrometría de Masas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2017 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Países Bajos