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Micro-CT vs. Whole Body Multirow Detector CT for Analysing Bone Regeneration in an Animal Model.
Bissinger, Oliver; Kirschke, Jan S; Probst, Florian Andreas; Stauber, Martin; Wolff, Klaus-Dietrich; Haller, Bernhard; Götz, Carolin; Plank, Christian; Kolk, Andreas.
Afiliación
  • Bissinger O; Department of Oral- and Maxillofacial Surgery, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany.
  • Kirschke JS; Department of Diagnostic and Interventional Radiology, Section of Neuroradiology, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany.
  • Probst FA; Department of Oral- and Maxillofacial Surgery, Ludwig-Maximilians-University of Munich, Munich, Germany.
  • Stauber M; Scanco Medical AG, Brüttisellen, Switzerland.
  • Wolff KD; Department of Oral- and Maxillofacial Surgery, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany.
  • Haller B; Institute of Medical Statistics and Epidemiology, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany.
  • Götz C; Department of Oral- and Maxillofacial Surgery, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany.
  • Plank C; Institute of Molecular Immunology - Experimental Oncology, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany.
  • Kolk A; Department of Oral- and Maxillofacial Surgery, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany.
PLoS One ; 11(11): e0166540, 2016.
Article en En | MEDLINE | ID: mdl-27880788
OBJECTIVES: Compared with multirow detector CT (MDCT), specimen (ex vivo) micro-CT (µCT) has a significantly higher (~ 30 x) spatial resolution and is considered the gold standard for assessing bone above the cellular level. However, it is expensive and time-consuming, and when applied in vivo, the radiation dose accumulates considerably. The aim of this study was to examine whether the lower resolution of the widely used MDCT is sufficient to qualitatively and quantitatively evaluate bone regeneration in rats. METHODS: Forty critical-size defects (5mm) were placed in the mandibular angle of rats and covered with coated bioactive titanium implants to promote bone healing. Five time points were selected (7, 14, 28, 56 and 112 days). µCT and MDCT were used to evaluate the defect region to determine the bone volume (BV), tissue mineral density (TMD) and bone mineral content (BMC). RESULTS: MDCT constantly achieved higher BV values than µCT (10.73±7.84 mm3 vs. 6.62±4.96 mm3, p<0.0001) and consistently lower TMD values (547.68±163.83 mm3 vs. 876.18±121.21 mm3, p<0.0001). No relevant difference was obtained for BMC (6.48±5.71 mm3 vs. 6.15±5.21 mm3, p = 0.40). BV and BMC showed very strong correlations between both methods, whereas TMD was only moderately correlated (r = 0.87, r = 0.90, r = 0.68, p < 0.0001). CONCLUSIONS: Due to partial volume effects, MDCT overestimated BV and underestimated TMD but accurately determined BMC, even in small volumes, compared with µCT. Therefore, if bone quantity is a sufficient end point, a considerable number of animals and costs can be saved, and compared with in vivo µCT, the required dose of radiation can be reduced.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Huesos / Regeneración Ósea / Microtomografía por Rayos X Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Huesos / Regeneración Ósea / Microtomografía por Rayos X Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos