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The Chromatin Remodeler ISW1 Is a Quality Control Factor that Surveys Nuclear mRNP Biogenesis.
Babour, Anna; Shen, Qingtang; Dos-Santos, Julien; Murray, Struan; Gay, Alexandre; Challal, Drice; Fasken, Milo; Palancade, Benoît; Corbett, Anita; Libri, Domenico; Mellor, Jane; Dargemont, Catherine.
Afiliación
  • Babour A; Université Paris Diderot, Sorbonne Paris Cité, INSERM UMR944, CNRS UMR7212, Hôpital St. Louis 1, Avenue Claude Vellefaux, 75475 Paris Cedex, France. Electronic address: anna.babour@inserm.fr.
  • Shen Q; Université Paris Diderot, Sorbonne Paris Cité, INSERM UMR944, CNRS UMR7212, Hôpital St. Louis 1, Avenue Claude Vellefaux, 75475 Paris Cedex, France.
  • Dos-Santos J; Université Paris Diderot, Sorbonne Paris Cité, INSERM UMR944, CNRS UMR7212, Hôpital St. Louis 1, Avenue Claude Vellefaux, 75475 Paris Cedex, France.
  • Murray S; Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
  • Gay A; Université Paris Diderot, Sorbonne Paris Cité, INSERM UMR944, CNRS UMR7212, Hôpital St. Louis 1, Avenue Claude Vellefaux, 75475 Paris Cedex, France.
  • Challal D; Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, CNRS, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris Cedex, France.
  • Fasken M; Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.
  • Palancade B; Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, CNRS, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris Cedex, France.
  • Corbett A; Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.
  • Libri D; Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, CNRS, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris Cedex, France.
  • Mellor J; Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
  • Dargemont C; Université Paris Diderot, Sorbonne Paris Cité, INSERM UMR944, CNRS UMR7212, Hôpital St. Louis 1, Avenue Claude Vellefaux, 75475 Paris Cedex, France.
Cell ; 167(5): 1201-1214.e15, 2016 11 17.
Article en En | MEDLINE | ID: mdl-27863241
Chromatin dynamics play an essential role in regulating DNA transaction processes, but it is unclear whether transcription-associated chromatin modifications control the mRNA ribonucleoparticles (mRNPs) pipeline from synthesis to nuclear exit. Here, we identify the yeast ISW1 chromatin remodeling complex as an unanticipated mRNP nuclear export surveillance factor that retains export-incompetent transcripts near their transcription site. This tethering activity of ISW1 requires chromatin binding and is independent of nucleosome sliding activity or changes in RNA polymerase II processivity. Combination of in vivo UV-crosslinking and genome-wide RNA immunoprecipitation assays show that Isw1 and its cofactors interact directly with premature mRNPs. Our results highlight that the concerted action of Isw1 and the nuclear exosome ensures accurate surveillance mechanism that proofreads the efficiency of mRNA biogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribonucleoproteínas / Saccharomyces cerevisiae / Adenosina Trifosfatasas / Proteínas de Saccharomyces cerevisiae / Proteínas de Unión al ADN Tipo de estudio: Prognostic_studies Idioma: En Revista: Cell Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribonucleoproteínas / Saccharomyces cerevisiae / Adenosina Trifosfatasas / Proteínas de Saccharomyces cerevisiae / Proteínas de Unión al ADN Tipo de estudio: Prognostic_studies Idioma: En Revista: Cell Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos