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A novel method for assaying human regulatory T cell direct suppression of B cell effector function.
Weingartner, Elizabeth; Courneya, Jean-Paul; Keegan, Achsah; Golding, Amit.
Afiliación
  • Weingartner E; Baltimore VA Medical Center, 10 N Greene St, Baltimore, MD 21201, United States; Department of Medicine, University of Maryland School of Medicine, Division of Rheumatology and Clinical Immunology, 10 S. Pine Street/MSTF Room 8-34, Baltimore, MD 21201, United States. Electronic address: eweingartner
  • Courneya JP; Baltimore VA Medical Center, 10 N Greene St, Baltimore, MD 21201, United States; Department of Medicine, University of Maryland School of Medicine, Division of Rheumatology and Clinical Immunology, 10 S. Pine Street/MSTF Room 8-34, Baltimore, MD 21201, United States. Electronic address: jpcourneya@h
  • Keegan A; Baltimore VA Medical Center, 10 N Greene St, Baltimore, MD 21201, United States; Department of Medicine, University of Maryland School of Medicine, Division of Rheumatology and Clinical Immunology, 10 S. Pine Street/MSTF Room 8-34, Baltimore, MD 21201, United States. Electronic address: akeegan@som.
  • Golding A; Baltimore VA Medical Center, 10 N Greene St, Baltimore, MD 21201, United States; Department of Medicine, University of Maryland School of Medicine, Division of Rheumatology and Clinical Immunology, 10 S. Pine Street/MSTF Room 8-34, Baltimore, MD 21201, United States. Electronic address: agolding@med
J Immunol Methods ; 441: 1-7, 2017 02.
Article en En | MEDLINE | ID: mdl-27851889
We have established a highly reproducible and reliable protocol for testing human regulatory T cell function in suppressing IgM production from an immature human B cell line. The autoreactive Ramos B cell line provides a stable reporter of B cell effector function that can be tested by a straight-forward IgM ELISA. Tregs from healthy volunteers display a range of ability for suppressing baseline IgM production in a contact- and death-independent manner. Having established the normal range for human Treg direct suppression of B cell effector function, it will now be possible to efficiently test Tregs from various autoimmune conditions in which B cell hyperactivity and secretion of auto-antibodies are a hallmark of disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Subgrupos de Linfocitos B / Técnicas Inmunológicas / Linfocitos T Reguladores Límite: Humans Idioma: En Revista: J Immunol Methods Año: 2017 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Subgrupos de Linfocitos B / Técnicas Inmunológicas / Linfocitos T Reguladores Límite: Humans Idioma: En Revista: J Immunol Methods Año: 2017 Tipo del documento: Article Pais de publicación: Países Bajos