Your browser doesn't support javascript.
loading
An automated approach to prepare tissue-derived spatially barcoded RNA-sequencing libraries.
Jemt, Anders; Salmén, Fredrik; Lundmark, Anna; Mollbrink, Annelie; Fernández Navarro, José; Ståhl, Patrik L; Yucel-Lindberg, Tülay; Lundeberg, Joakim.
Afiliación
  • Jemt A; Science for Life Laboratory, Department of Gene Technology, Royal Institute of Technology, SE-106 91, Stockholm, Sweden.
  • Salmén F; Science for Life Laboratory, Department of Gene Technology, Royal Institute of Technology, SE-106 91, Stockholm, Sweden.
  • Lundmark A; Department of Dental Medicine, Division of Periodontology, Karolinska Institutet, SE-141 04, Huddinge, Sweden.
  • Mollbrink A; Science for Life Laboratory, Department of Gene Technology, Royal Institute of Technology, SE-106 91, Stockholm, Sweden.
  • Fernández Navarro J; Science for Life Laboratory, Department of Gene Technology, Royal Institute of Technology, SE-106 91, Stockholm, Sweden.
  • Ståhl PL; Department of Cell and Molecular Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
  • Yucel-Lindberg T; Department of Dental Medicine, Division of Periodontology, Karolinska Institutet, SE-141 04, Huddinge, Sweden.
  • Lundeberg J; Science for Life Laboratory, Department of Gene Technology, Royal Institute of Technology, SE-106 91, Stockholm, Sweden.
Sci Rep ; 6: 37137, 2016 11 16.
Article en En | MEDLINE | ID: mdl-27849009
Sequencing the nucleic acid content of individual cells or specific biological samples is becoming increasingly common. This drives the need for robust, scalable and automated library preparation protocols. Furthermore, an increased understanding of tissue heterogeneity has lead to the development of several unique sequencing protocols that aim to retain or infer spatial context. In this study, a protocol for retaining spatial information of transcripts has been adapted to run on a robotic workstation. The method spatial transcriptomics is evaluated in terms of robustness and variability through the preparation of reference RNA, as well as through preparation and sequencing of six replicate sections of a gingival tissue biopsy from a patient with periodontitis. The results are reduced technical variability between replicates and a higher throughput, processing four times more samples with less than a third of the hands on time, compared to the standard protocol.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periodontitis / ARN / Biblioteca de Genes / Automatización de Laboratorios / Código de Barras del ADN Taxonómico / Encía Límite: Humans Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periodontitis / ARN / Biblioteca de Genes / Automatización de Laboratorios / Código de Barras del ADN Taxonómico / Encía Límite: Humans Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido