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Age-Associated B Cells with Proinflammatory Characteristics Are Expanded in a Proportion of Multiple Sclerosis Patients.
Claes, Nele; Fraussen, Judith; Vanheusden, Marjan; Hellings, Niels; Stinissen, Piet; Van Wijmeersch, Bart; Hupperts, Raymond; Somers, Veerle.
Afiliación
  • Claes N; Biomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, 3590 Diepenbeek, Belgium.
  • Fraussen J; Biomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, 3590 Diepenbeek, Belgium.
  • Vanheusden M; Biomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, 3590 Diepenbeek, Belgium.
  • Hellings N; Biomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, 3590 Diepenbeek, Belgium.
  • Stinissen P; Biomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, 3590 Diepenbeek, Belgium.
  • Van Wijmeersch B; Biomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, 3590 Diepenbeek, Belgium.
  • Hupperts R; Rehabilitation and MS-Center, B-3900 Overpelt, Belgium.
  • Somers V; Department of Neuroscience, School of Mental Health and Neuroscience, Maastricht University, 6229 ER Maastricht, the Netherlands; and.
J Immunol ; 197(12): 4576-4583, 2016 12 15.
Article en En | MEDLINE | ID: mdl-27837111
Immune aging occurs in the elderly and in autoimmune diseases. Recently, IgD-CD27- (double negative, DN) and CD21-CD11c+ (CD21low) B cells were described as age-associated B cells with proinflammatory characteristics. This study investigated the prevalence and functional characteristics of DN and CD21low B cells in multiple sclerosis (MS) patients. Using flow cytometry, we demonstrated a higher proportion of MS patients younger than 60 y with peripheral expansions of DN (8/41) and CD21low (9/41) B cells compared with age-matched healthy donors (1/33 and 2/33, respectively), which indicates an increase in age-associated B cells in MS patients. The majority of DN B cells had an IgG+ memory phenotype, whereas CD21low B cells consisted of a mixed population of CD27- naive, CD27+ memory, IgG+, and IgM+ cells. DN B cells showed similar (MS patients) or increased (healthy donors) MHC-II expression as class-switched memory B cells and intermediate costimulatory molecule expression between naive and class-switched memory B cells, indicating their potential to induce (proinflammatory) T cell responses. Further, DN B cells produced proinflammatory and cytotoxic cytokines following ex vivo stimulation. Increased frequencies of DN and CD21low B cells were found in the cerebrospinal fluid of MS patients compared with paired peripheral blood. In conclusion, a proportion of MS patients showed increased peripheral expansions of age-associated B cells. DN and CD21low B cell frequencies were further increased in MS cerebrospinal fluid. These cells could contribute to inflammation by induction of T cell responses and the production of proinflammatory cytokines.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Envejecimiento / Linfocitos B / Linfocitos T / Subgrupos de Linfocitos B / Esclerosis Múltiple Tipo de estudio: Risk_factors_studies Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: J Immunol Año: 2016 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Estados Unidos
Buscar en Google
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Envejecimiento / Linfocitos B / Linfocitos T / Subgrupos de Linfocitos B / Esclerosis Múltiple Tipo de estudio: Risk_factors_studies Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: J Immunol Año: 2016 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Estados Unidos