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Leishmania infantum INFECTION IN DOGS FROM THE SOUTHERN REGION OF MINAS GERAIS STATE, BRAZIL.
Nunes, Juliana Barbosa; Laurenti, Márcia Dalastra; Kanamura, Herminia Yohko; Pereira, Alessandro Antônio Costa; Colombo, Fabio Antonio; Marques, Marcos José.
Afiliación
  • Nunes JB; Federal University of Alfenas, Institute of Biomedical Sciences. Alfenas, MG, Brazil.
  • Laurenti MD; University of São Paulo, College of Medicine, Department of Pathology. São Paulo, SP, Brazil.
  • Kanamura HY; Federal University of Alfenas, Institute of Biomedical Sciences. Alfenas, MG, Brazil.
  • Pereira AA; Federal University of Alfenas, Institute of Biomedical Sciences. Alfenas, MG, Brazil.
  • Colombo FA; Federal University of Alfenas, Institute of Biomedical Sciences. Alfenas, MG, Brazil.
  • Marques MJ; Federal University of Alfenas, Institute of Biomedical Sciences. Alfenas, MG, Brazil.
Rev Inst Med Trop Sao Paulo ; 58: 75, 2016 Nov 03.
Article en En | MEDLINE | ID: mdl-27828616
Visceral leishmaniasis is a systemic and chronic disease and dogs are the main reservoir of the etiologic agent, Leishmania infantum (syn L. chagasi). A serological and molecular investigation of canine visceral leishmaniasis (CVL) was performed in the municipality of Alfenas, located in the southern region of Minas Gerais, where the disease is not endemic. Samples from 87 dogs were submitted to serological tests including the Dual Path Platform (DPP (r) ) CVL Bio-Manguinhos rapid test, an in-house enzyme-linked immunosorbent assay (ELISA) and an immunofluorescence antibody test (IFAT), as well as molecular techniques such as a conventional polymerase chain reaction (PCR) with the RV1/RV2 primers and a quantitative PCR (qPCR) with the LinJ31, Ldon and DNApol primers. Of the 87 serum samples, eight (9.2%) were positive for Leishmania using the DPP rapid test, but only four (4.6%) were confirmed by ELISA and two (2.3%) by IFAT. In these two serologically confirmed cases, spleen and liver samples were positive by all the employed molecular and parasitological procedures performed on spleen samples. When whole blood samples were used in the molecular assays, two samples (2.3%) were positive only by qPCR. DNA extracted and amplified from the spleens of seropositive dogs was sequenced, showing 100% of similarity with the Leishmania infantum (syn L. chagasi) sequence. Thus, the first cases of CVL have been confirmed in the Alfenas region, suggesting the importance of canine surveys in non-endemic municipalities for CVL to monitor disease progression and to prevent outbreaks.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anticuerpos Antiprotozoarios / Leishmania infantum / Enfermedades de los Perros / Leishmaniasis Visceral Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Rev Inst Med Trop Sao Paulo Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anticuerpos Antiprotozoarios / Leishmania infantum / Enfermedades de los Perros / Leishmaniasis Visceral Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Rev Inst Med Trop Sao Paulo Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil