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Enzyme-linked, aptamer-based, competitive biolayer interferometry biosensor for palytoxin.
Gao, Shunxiang; Zheng, Xin; Hu, Bo; Sun, Mingjuan; Wu, Jihong; Jiao, Binghua; Wang, Lianghua.
Afiliación
  • Gao S; Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China; Research Center, Eye & ENT Hospital, Fudan University, Shanghai, 200032 China; Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai 2
  • Zheng X; Department of Laboratory Diagnosis, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
  • Hu B; Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China; Marine Biological Institute, College of Marine Military Medicine, Second Military Medical University, Shanghai 200433, China.
  • Sun M; Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China.
  • Wu J; Research Center, Eye & ENT Hospital, Fudan University, Shanghai, 200032 China; Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai 200032, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, Shanghai 200032, China. Electronic address: jihongwu@fudan.edu.cn.
  • Jiao B; Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China; Marine Biological Institute, College of Marine Military Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: bhjiao@sm
  • Wang L; Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China. Electronic address: lhwang@smmu.edu.cn.
Biosens Bioelectron ; 89(Pt 2): 952-958, 2017 Mar 15.
Article en En | MEDLINE | ID: mdl-27816587
In this study, we coupled biolayer interferometry (BLI) with competitive binding assay through an enzyme-linked aptamer and developed a real-time, ultra-sensitive, rapid quantitative method for detection of the marine biotoxin palytoxin. Horseradish peroxidase-labeled aptamers were used as biorecognition receptors to competitively bind with palytoxin, which was immobilized on the biosensor surface. The palytoxin: horseradish peroxidase-aptamer complex was then submerged in a 3,3'-diaminobenzidine solution, which resulted in formation of a precipitated polymeric product directly on the biosensor surface and a large change in the optical thickness of the biosensor layer. This change could obviously shift the interference pattern and generate a response profile on the BLI biosensor. The biosensor showed a broad linear range for palytoxin (200-700pg/mL) with a low detection limit (0.04pg/mL). Moreover, the biosensor was applied to the detection of palytoxin in spiked extracts and showed a high degree of selectivity for palytoxin, good reproducibility, and stability. This enzyme-linked, aptamer-based, competitive BLI biosensor offers a promising method for rapid and sensitive detection of palytoxin and other analytes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acrilamidas / Técnicas Biosensibles / Aptámeros de Nucleótidos Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acrilamidas / Técnicas Biosensibles / Aptámeros de Nucleótidos Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido