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Estimation of genetic diversity in a natural population of cambui tree (Myrciaria tenella O. Berg) using ISSR markers.
Santana, J G S; Nascimento, A L S; Costa, T S; de Almeida, T M B; Rabbani, A R C; Silva, A V C.
Afiliación
  • Santana JG; Programa de Pós-Graduação em Agricultura e Biodiversidade, Universidade Federal de Sergipe, São Cristovão, SE, Brasil.
  • Nascimento AL; Embrapa Tabuleiros Costeiros, Aracaju, SE, Brasil.
  • Costa TS; Embrapa Tabuleiros Costeiros, Aracaju, SE, Brasil.
  • de Almeida TM; Embrapa Tabuleiros Costeiros, Aracaju, SE, Brasil.
  • Rabbani AR; Instituto Federal da Bahia, Porto Seguro, BA, Brasil.
  • Silva AV; Embrapa Tabuleiros Costeiros, Aracaju, SE, Brasil ana.veruska@embrapa.br.
Genet Mol Res ; 15(4)2016 Oct 05.
Article en En | MEDLINE | ID: mdl-27808378
Cambui (Myrciaria tenella O. Berg) is a native species from Brazil, which belongs to the family Myrtaceae. Molecular characterization is one of the most used tools for the study of the biotechnological potential of species because the diversity level between individuals can be inferred. Analysis of genetic diversity is fundamental to the direction of the strategies necessary to form and maintain a germplasm. This study aimed to evaluate the genetic diversity in a natural population of cambui using inter-simple sequence repeat (ISSR) molecular markers. The natural population, which provided the plant material, is found at the Private Reserve of Natural Heritage of Caju, which belongs to the experimental field of Embrapa Tabuleiros Costeiros, in the municipality of Itaporanga d'Ajuda, SE, Brazil. Young leaves of each individual were collected for DNA extraction and analysis of PCR-ISSR. Thirty primers were tested and the top 10 were selected. The use of these primers resulted in 71 fragments with 98.3% polymorphism. Similarity of individuals ranged between 0.30 and 0.92. The most similar individuals were C13 and C17 and the most distant were C1 and C41. Through UPGMA, six distinct groups were identified. This information may be used for conservation of these genetic resources, germplasm exchange, creation of germplasm bank and in future studies with this species.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Árboles / Variación Genética / Repeticiones de Microsatélite Tipo de estudio: Prognostic_studies Idioma: En Revista: Genet Mol Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Árboles / Variación Genética / Repeticiones de Microsatélite Tipo de estudio: Prognostic_studies Idioma: En Revista: Genet Mol Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil