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Klotho mitigates cyclosporine A (CsA)-induced epithelial-mesenchymal transition (EMT) and renal fibrosis in rats.
Liu, Qi-Feng; Ye, Jian-Ming; Yu, Li-Xia; Dong, Xiao-Hong; Feng, Jian-Hua; Xiong, Yan; Gu, Xiao-Xia; Li, Sha-Sha.
Afiliación
  • Liu QF; Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
  • Ye JM; Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
  • Yu LX; Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
  • Dong XH; Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
  • Feng JH; Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
  • Xiong Y; Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
  • Gu XX; Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
  • Li SS; Clinical Research Centre, Kunshan First People's Hospital Affiliated to Jiangsu University, 91 Qianjin West Road, Kunshan, Jiangsu, China. whitelss@163.com.
Int Urol Nephrol ; 49(2): 345-352, 2017 Feb.
Article en En | MEDLINE | ID: mdl-27796696
PURPOSE: Klotho deficiency is implicated in various kidney diseases, including renal fibrosis. The aim of this study was to investigate the effect of Klotho administration on epithelial-mesenchymal transition (EMT) and renal fibrosis induced by cyclosporine A (CsA) in rats. METHODS: CsA-induced renal fibrosis was established by oral administration of CsA (30 mg/kg) to rats on a low-salt diet for 28 days. Klotho was administered to rats by intraperitoneal injection. Renal pathological changes were evaluated by hematoxylin and eosin and Masson's trichrome staining. The EMT response was assessed by measuring the level of TGF-ß1, E-cadherin and α-SMA by immunohistochemistry and Western blot. RESULTS: Administration of CsA for 28 days induced renal damage, decreased Klotho expression and activated the EMT response (demonstrated as increased TGF-ß1 and α-SMA expression accompanied by decreased in E-cadherin expression). Treatment with Klotho significantly ameliorated pathological lesions of the kidney by modulating the expression of EMT-associated proteins in the kidney. CONCLUSIONS: Klotho inhibits CsA-induced EMT and renal fibrosis in rats. Klotho may serve as a therapeutic agent to minimize CsA-induced renal fibrosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transición Epitelial-Mesenquimal / Glucuronidasa / Enfermedades Renales Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int Urol Nephrol Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transición Epitelial-Mesenquimal / Glucuronidasa / Enfermedades Renales Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int Urol Nephrol Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos