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Energy of Liposome Patch Adhesion to the Pipet Glass Determined by Confocal Fluorescence Microscopy.
Nakayama, Yoshitaka; Slavchov, Radomir I; Bavi, Navid; Martinac, Boris.
Afiliación
  • Nakayama Y; Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute , Darlinghurst, New South Wales 2010, Australia.
  • Slavchov RI; Department of Physical Chemistry, Sofia University , 1 J. Bourchier Blvd., Sofia 1126, Bulgaria.
  • Bavi N; Department of Chemical Engineering and Biotechnology, Cambridge University , Pembroke Street, New Museums Site, CB2 3RA Cambridge, United Kingdom.
  • Martinac B; Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute , Darlinghurst, New South Wales 2010, Australia.
J Phys Chem Lett ; 7(22): 4530-4534, 2016 Nov 17.
Article en En | MEDLINE | ID: mdl-27791368
The formation of the gigaseal in the patch clamp technique is dependent on the adhesion between the cell or liposome membrane and the glass pipet. The adhesion results in a capillary force causing creep of the patch membrane up the pipet. The membrane can be immobilized by counteracting the capillary force by positive pressure applied to the patch pipet. We use this phenomenon to develop a method for static measurement of the adhesion free energy of the lipid bilayer to the glass. Confocal fluorescent microscopy is used to track the bilayer creep inside the pipet and measure the immobilization pressure at various salt concentrations and pH. The adhesion energy is simply related to this pressure. For the studied phospholipid bilayers, its values were in the 0.3-0.7 mJ/m2 range, increased with salt concentration, and had a maximum as a function of pH. This method offers a way to measure bilayer-glass adhesion energy in patch clamp experiments that is more precise than dynamic methods.
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Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Phys Chem Lett Año: 2016 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Buscar en Google
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Phys Chem Lett Año: 2016 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos