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Pyrazinamide susceptibility testing in Mycobacterium tuberculosis using the fast resazurin microtiter assay plate.
Pina, R Z; Caleffi-Ferracioli, K R; Campanerut-Sá, P A Z; Ghiraldi-Lopez, L D; Pavan, F R; Siqueira, V L D; Scodro, R B L; Cardoso, R F.
Afiliación
  • Pina RZ; Post-Graduate Programme in Health Sciences, State University of Maringá, Maringá, Brazil.
  • Caleffi-Ferracioli KR; Department of Clinical Analyses and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil.
  • Campanerut-Sá PA; Department of Clinical Analyses and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil.
  • Ghiraldi-Lopez LD; Department of Clinical Analyses and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil.
  • Pavan FR; School of Pharmaceutical Science, São Paulo State University, Araraquara, São Paulo, São Paulo, Brazil.
  • Siqueira VL; Department of Clinical Analyses and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil.
  • Scodro RB; Department of Clinical Analyses and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil.
  • Cardoso RF; Department of Clinical Analyses and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil.
Int J Tuberc Lung Dis ; 20(11): 1535-1538, 2016 11.
Article en En | MEDLINE | ID: mdl-27776597
SETTING: Department of Clinical Analysis and Biomedicine, State University of Maringa, Maringa, PR, Brazil. OBJECTIVE: To evaluate the performance of the resazurin microtiter assay (REMA) plate at pH 5.5 in detecting Mycobacterium tuberculosis susceptibility to pyrazinamide (PZA). DESIGN: The minimal inhibitory concentration (MIC) of PZA in M. tuberculosis H37Rv and M. bovis AN5 reference strains and in 34 clinical M. tuberculosis isolates (26 PZA-susceptible and eight PZA-resistant) was determined using REMA at pH 5.5 and compared to REMA at pH 6.0. RESULTS: REMA at pH 5.5 was helpful in discriminating PZA-susceptible from resistant M. tuberculosis isolates when â©¿50 µg/ml PZA was considered as the cut-off for PZA susceptibility. Furthermore, it provided results in 8 days. However, two PZA-resistant isolates failed to grow at pH 5.5. CONCLUSION: As the REMA method is rapid, inexpensive, easy to perform and read, it would be of great usefulness in low-income countries for detecting PZA-resistant M. tuberculosis. REMA at pH 5.6-5.9 should be evaluated on an extended panel of clinical M. tuberculosis isolates with a greater range of MIC values in different laboratories for a better understanding of its utility in differentiating PZA-resistant from PZA-susceptible isolates.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pirazinamida / Pruebas de Sensibilidad Microbiana / Farmacorresistencia Bacteriana / Mycobacterium tuberculosis / Antituberculosos Límite: Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Int J Tuberc Lung Dis Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Francia
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pirazinamida / Pruebas de Sensibilidad Microbiana / Farmacorresistencia Bacteriana / Mycobacterium tuberculosis / Antituberculosos Límite: Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Int J Tuberc Lung Dis Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Francia