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Novel bead-based platform for direct detection of unlabelled nucleic acids through Single Nucleobase Labelling.
Venkateswaran, Seshasailam; Luque-González, Maria Angélica; Tabraue-Chávez, Mavys; Fara, Mario Antonio; López-Longarela, Barbara; Cano-Cortes, Victoria; López-Delgado, Francisco Javier; Sánchez-Martín, Rosario María; Ilyine, Hugh; Bradley, Mark; Pernagallo, Salvatore; Díaz-Mochón, Juan José.
Afiliación
  • Venkateswaran S; School of Chemistry, EaStCHEM, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3FJ, United Kingdom.
  • Luque-González MA; Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Parque Tecnológico de Ciencias de la Salud (PTS), Avenida de la Ilustración 114, Granada 18016, Spain; Faculty of Pharmacy, University of Granada, Campus Cartuja s/n, Granada 18071, Spain.
  • Tabraue-Chávez M; DestiNA Genomica S.L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, Armilla, Granada 18016, Spain.
  • Fara MA; DestiNA Genomica S.L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, Armilla, Granada 18016, Spain; DestiNA Genomics Ltd., 7-11 Melville St, Edinburgh, EH3 7PE United Kingdom.
  • López-Longarela B; DestiNA Genomica S.L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, Armilla, Granada 18016, Spain.
  • Cano-Cortes V; Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Parque Tecnológico de Ciencias de la Salud (PTS), Avenida de la Ilustración 114, Granada 18016, Spain; Faculty of Pharmacy, University of Granada, Campus Cartuja s/n, Granada 18071, Spain.
  • López-Delgado FJ; Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Parque Tecnológico de Ciencias de la Salud (PTS), Avenida de la Ilustración 114, Granada 18016, Spain; Faculty of Pharmacy, University of Granada, Campus Cartuja s/n, Granada 18071, Spain.
  • Sánchez-Martín RM; Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Parque Tecnológico de Ciencias de la Salud (PTS), Avenida de la Ilustración 114, Granada 18016, Spain; Faculty of Pharmacy, University of Granada, Campus Cartuja s/n, Granada 18071, Spain.
  • Ilyine H; DestiNA Genomics Ltd., 7-11 Melville St, Edinburgh, EH3 7PE United Kingdom.
  • Bradley M; School of Chemistry, EaStCHEM, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3FJ, United Kingdom.
  • Pernagallo S; DestiNA Genomica S.L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, Armilla, Granada 18016, Spain; DestiNA Genomics Ltd., 7-11 Melville St, Edinburgh, EH3 7PE United Kingdom. Electronic address: salvatore@destinagenomics.com.
  • Díaz-Mochón JJ; Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Parque Tecnológico de Ciencias de la Salud (PTS), Avenida de la Ilustración 114, Granada 18016, Spain; Faculty of Pharmacy, University of Granada, Campus Cartuja s/n, Granada 18071, Spain; DestiNA
Talanta ; 161: 489-496, 2016 Dec 01.
Article en En | MEDLINE | ID: mdl-27769437
Over the last decade, circulating microRNAs have received attention as diagnostic and prognostic biomarkers. In particular, microRNA122 has been demonstrated to be an early and more sensitive indicator of drug-induced liver injury than the widely used biomarkers such as alanine aminotransferase and aspartate aminotransferase. Recently, microRNA122 has been used in vitro to assess the cellular toxicity of new drugs and as a biomarker for the development of a rapid test for drug overdose/liver damage. In this proof-of-concept study, we report a PCR-free and label-free detection method that has a limit of detection (3 standard deviations) of 15 fmoles of microRNA122, by integrating a dynamic chemical approach for "Single Nucleobase Labelling" with a bead-based platform (Luminex®) thereby, in principle, demonstrating the exciting prospect of rapid and accurate profiling of any microRNAs related to diseases and toxicology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs Tipo de estudio: Diagnostic_studies Idioma: En Revista: Talanta Año: 2016 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs Tipo de estudio: Diagnostic_studies Idioma: En Revista: Talanta Año: 2016 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Países Bajos