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Fabrication, characterization and application of laccase-nylon 6,6/Fe3+ composite nanofibrous membrane for 3,3'-dimethoxybenzidine detoxification.
Jasni, M Jasmin Fathi; Sathishkumar, Palanivel; Sornambikai, Sundaram; Yusoff, Abdull Rahim Mohd; Ameen, Fuad; Buang, Nor Aziah; Kadir, Mohammed Rafiq Abdul; Yusop, Zulkifli.
Afiliación
  • Jasni MJ; Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Malaysia.
  • Sathishkumar P; Centre for Sustainable Environment and Water Security (IPASA), Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia. salemsathishkumar@gmail.com.
  • Sornambikai S; Medical Devices and Technology Group (MEDITEG), Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia.
  • Yusoff AR; Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Malaysia. rahim@kimia.fs.utm.my.
  • Ameen F; Centre for Sustainable Nanomaterials, Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia. rahim@kimia.fs.utm.my.
  • Buang NA; Department of Botany and Microbiology, Faculty of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
  • Kadir MR; Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Malaysia.
  • Yusop Z; Medical Devices and Technology Group (MEDITEG), Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia.
Bioprocess Biosyst Eng ; 40(2): 191-200, 2017 Feb.
Article en En | MEDLINE | ID: mdl-27757535
In this study, laccase was immobilized on nylon 6,6/Fe3+ composite (NFC) nanofibrous membrane and used for the detoxification of 3,3'-dimethoxybenzidine (DMOB). The average size and tensile strength of the NFC membrane were found to be 60-80 nm (diameter) and 2.70 MPa, respectively. The FTIR results confirm that the amine (N-H) group of laccase was attached with Fe3+ particles and the carbonyl (C=O) group of NFC membrane via hydrogen bonding. The half-life of the laccase-NFC membrane storage stability was increased from 6 to 11 weeks and the reusability was significantly extended up to 43 cycles against ABTS oxidation. Enhanced electro-oxidation of DMOB by laccase was observed at 0.33 V and the catalytic current was found to be 30 µA. The DMOB-treated mouse fibroblast 3T3-L1 preadipocytes showed maximum (97 %) cell inhibition at 75 µM L-1 within 24 h. The cytotoxicity of DMOB was significantly decreased to 78 % after laccase treatment. This study suggests that laccase-NFC membrane might be a good candidate for emerging pollutant detoxification.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Caprolactama / Proteínas Fúngicas / Compuestos Férricos / Lacasa / Dianisidina / Trametes / Nanofibras / Membranas Artificiales Límite: Animals Idioma: En Revista: Bioprocess Biosyst Eng Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2017 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Caprolactama / Proteínas Fúngicas / Compuestos Férricos / Lacasa / Dianisidina / Trametes / Nanofibras / Membranas Artificiales Límite: Animals Idioma: En Revista: Bioprocess Biosyst Eng Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2017 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Alemania