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Permeability Profiles and Intestinal Toxicity Assessment of Hydrochlorothiazide and Its Inclusion Complex with ß-Cyclodextrin Loaded into Chitosan Nanoparticles.
Onnainty, R; Schenfeld, E M; Petiti, J P; Longhi, M R; Torres, A; Quevedo, M A; Granero, G E.
Afiliación
  • Onnainty R; Departamento de Farmacia, UNITEFA, CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba , Córdoba, X5000HUA, Argentina.
  • Schenfeld EM; Departamento de Farmacia, UNITEFA, CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba , Córdoba, X5000HUA, Argentina.
  • Petiti JP; Centro de Microscopía Electrónica, UNC, INICSA, CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba , Córdoba, X5000HUA, Argentina.
  • Longhi MR; Departamento de Farmacia, UNITEFA, CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba , Córdoba, X5000HUA, Argentina.
  • Torres A; Centro de Microscopía Electrónica, UNC, INICSA, CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba , Córdoba, X5000HUA, Argentina.
  • Quevedo MA; Departamento de Farmacia, UNITEFA, CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba , Córdoba, X5000HUA, Argentina.
  • Granero GE; Departamento de Farmacia, UNITEFA, CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba , Córdoba, X5000HUA, Argentina.
Mol Pharm ; 13(11): 3736-3746, 2016 11 07.
Article en En | MEDLINE | ID: mdl-27756134
Here, a novel drug delivery system was developed for the hydrochlorothiazide (HCT):ß-cyclodextrin (ßCD) inclusion complex loaded into chitosan (CS) nanoparticles (NPs) [CS/HCT:ßCD NPs]. It was found, for the first time, that exposure of the intestinal mucosa to free HCT resulted in an increased and abnormal intestinal permeability associated with several injuries to the intestinal epithelium. Nevertheless, the HCT delivery system obtained ameliorated the damage of the intestinal epithelium induced by HCT. Furthermore, we found that the corresponding permeability profiles for both the free HCT and the CS/HCT:ßCD NPs were exponential and lineal, respectively. We propose that the increased intestinal uptake and severe tissue injury of HCT to the intestinal epithelium could be directly related to possible effects of this drug on the ionoregulatory Na+/K+-ATPase channel. Thus, it is postulated that the CS/HCT:ßCD NPs may increase the gastrointestinal retention of the HCT, which would provide increased adherence to the mucus barrier that lines the intestinal epithelium; consequently, this would act as a slow HCT release delivery system and maintain lower drug levels of luminal gut in comparison with the administration of free HCT, leading to less severe local injury.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Beta-Ciclodextrinas / Quitosano / Nanopartículas / Hidroclorotiazida Límite: Animals Idioma: En Revista: Mol Pharm Asunto de la revista: BIOLOGIA MOLECULAR / FARMACIA / FARMACOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Argentina Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Beta-Ciclodextrinas / Quitosano / Nanopartículas / Hidroclorotiazida Límite: Animals Idioma: En Revista: Mol Pharm Asunto de la revista: BIOLOGIA MOLECULAR / FARMACIA / FARMACOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Argentina Pais de publicación: Estados Unidos