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Ultrasound-assisted hydrolysis of waste cooking oil catalyzed by homemade lipases.
Mulinari, J; Venturin, B; Sbardelotto, M; Dall Agnol, A; Scapini, T; Camargo, A F; Baldissarelli, D P; Modkovski, T A; Rossetto, V; Dalla Rosa, C; Reichert, F W; Golunski, S M; Vieitez, I; Vargas, G D L P; Dalla Rosa, C; Mossi, A J; Treichel, H.
Afiliación
  • Mulinari J; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Venturin B; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Sbardelotto M; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Dall Agnol A; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Scapini T; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Camargo AF; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Baldissarelli DP; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Modkovski TA; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Rossetto V; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Dalla Rosa C; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Reichert FW; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Golunski SM; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Vieitez I; Grupo de Derivados de la Industria Alimentaria, Departamento de Ciencia y Tecnología de Alimentos (CYTAL), Facultad de Química, Universidad de la República (UdelaR), Montevideo, Uruguay.
  • Vargas GDLP; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Dalla Rosa C; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Mossi AJ; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil.
  • Treichel H; Environmental Science and Technology, Federal University of Fronteira Sul - Campus Erechim, RS 135, Km 72, 99700-000 Erechim, RS, Brazil. Electronic address: helentreichel@gmail.com.
Ultrason Sonochem ; 35(Pt A): 313-318, 2017 Mar.
Article en En | MEDLINE | ID: mdl-27746067
This study aimed to evaluate the waste cooking oil (WCO) hydrolysis in ultrasonic system using lipase as catalyst. Lipase was produced by the fungus Aspergillus niger via solid state fermentation (SSF) using canola meal as substrate. Prior to the hydrolysis reaction, the lipase behavior when subjected to ultrasound was evaluated by varying the temperature of the ultrasonic bath, the exposure time and the equipment power. Having optimized the treatment on ultrasound, the WCO hydrolysis reaction was carried out by evaluating the oil:water ratio and the lipase concentration. For a greater homogenization of the reaction medium, a mechanical stirrer at 170rpm was used. All steps were analyzed by experimental design technique. The lipase treatment in ultrasound generated an increase of about 320% in its hydrolytic activity using 50% of ultrasonic power for 25min. at 45°C. The results of the experimental design conducted for ultrasound-assisted hydrolysis showed that the best condition was using an oil:water ratio of 1:3 (v:v) and enzyme concentration of 15% (v/v), generating 62.67µmol/mL of free fatty acids (FFA) in 12h of reaction. Thus, the use of Aspergillus niger lipase as a catalyst for hydrolysis reaction of WCO can be considered as a possible pretreatment technique of the oil in order to accelerate its degradation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Residuos / Aceites / Culinaria / Biocatálisis / Ondas Ultrasónicas / Lipasa Idioma: En Revista: Ultrason Sonochem Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Residuos / Aceites / Culinaria / Biocatálisis / Ondas Ultrasónicas / Lipasa Idioma: En Revista: Ultrason Sonochem Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos