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MiR-181a-5p regulates 3T3-L1 cell adipogenesis by targeting Smad7 and Tcf7l2.
Ouyang, Dan; Xu, Lifeng; Zhang, Lihua; Guo, Dongguang; Tan, Xiaotong; Yu, Xiaofang; Qi, Junjie; Ye, Yaqiong; Liu, Qihong; Ma, Yongjiang; Li, Yugu.
Afiliación
  • Ouyang D; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
  • Xu L; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
  • Zhang L; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
  • Guo D; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
  • Tan X; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
  • Yu X; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
  • Qi J; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
  • Ye Y; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
  • Liu Q; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
  • Ma Y; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
  • Li Y; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China yuguli99@163.com liyugu@scau.edu.cn.
Acta Biochim Biophys Sin (Shanghai) ; 48(11): 1034-1041, 2016 Nov.
Article en En | MEDLINE | ID: mdl-27742678
MicroRNAs are highly conserved non-coding small RNAs participating in almost all kinds of biological activities. MiR-181a has been reported to be involved in the differentiation of porcine primary preadipocytes, but the profound effect of miR-181a-5p on 3T3-L1 adipocyte differentiation and proliferation is still unclear. In this study, we found that supplementation of miR-181a-5p in 3T3-L1 cells significantly promoted the adipogenesis and inhibited cell proliferation with increased expression of adipogenic marker genes including peroxisome proliferator-activated receptor gamma (Pparγ), CCAAT/enhancer-binding protein alpha (C/ebpα), fatty acid-binding protein 4 (Fabp4), and Adiponectin, accompanied by an accumulation of lipid droplet, an increase of triglyceride content, and a decrease of cell proliferation. Furthermore, by using the luciferase assay, Smad7 and Tcf7l2, two important members of transforming growth factor-ß (TGFß) and Wnt signaling pathway, were proven to be the direct target genes of miR-181a-5p. Moreover, supplementation of miR-181a-5p in 3T3-L1 cells altered the expressions of proteins involved in the TGFß signaling pathway, such as TGFBR1, p-SMAD3, SMAD4, c-MYC, and p15. Taken together, these results indicate that miR-181a-5p promotes 3T3-L1 preadipocyte differentiation and adipogenesis through regulating TGFß/Smad and Wnt signaling pathway by directly targeting Smad7 and Tcf7l2.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Proteína smad7 / Adipogénesis / Proteína 1 Similar al Factor de Transcripción 7 Límite: Animals Idioma: En Revista: Acta Biochim Biophys Sin (Shanghai) Asunto de la revista: BIOFISICA / BIOQUIMICA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Buscar en Google
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Proteína smad7 / Adipogénesis / Proteína 1 Similar al Factor de Transcripción 7 Límite: Animals Idioma: En Revista: Acta Biochim Biophys Sin (Shanghai) Asunto de la revista: BIOFISICA / BIOQUIMICA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: China