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Backbone assignment of the binary complex of the full length Sulfolobus solfataricus DNA polymerase IV and DNA.
Lee, Eunjeong; Fowler, Jason D; Suo, Zucai; Wu, Zhengrong.
Afiliación
  • Lee E; Department of Chemistry and Biochemistry, The Ohio State University, 876 Biological Sciences, 484 West 12th Ave., Columbus, OH, 43210, USA.
  • Fowler JD; Department of Chemistry and Biochemistry, The Ohio State University, 876 Biological Sciences, 484 West 12th Ave., Columbus, OH, 43210, USA.
  • Suo Z; Department of Chemistry and Biochemistry, The Ohio State University, 876 Biological Sciences, 484 West 12th Ave., Columbus, OH, 43210, USA.
  • Wu Z; Department of Chemistry and Biochemistry, The Ohio State University, 876 Biological Sciences, 484 West 12th Ave., Columbus, OH, 43210, USA. wu.473@osu.edu.
Biomol NMR Assign ; 11(1): 39-43, 2017 04.
Article en En | MEDLINE | ID: mdl-27738883
Sulfolobus solfataricus DNA polymerase IV (Dpo4), a model Y-family DNA polymerase, bypasses a wide range of DNA lesions in vitro and in vivo. In this paper, we report the backbone chemical shift assignments of the full length Dpo4 in its binary complex with a 14/14-mer DNA substrate. Upon DNA binding, several ß-stranded regions in the isolated catalytic core and little finger/linker fragments of Dpo4 become more structured. This work serves as a foundation for our ongoing investigation of conformational dynamics of Dpo4 and future determination of the first solution structures of a DNA polymerase and its binary and ternary complexes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / ADN Polimerasa beta / Resonancia Magnética Nuclear Biomolecular / Sulfolobus solfataricus Idioma: En Revista: Biomol NMR Assign Asunto de la revista: BIOLOGIA MOLECULAR / MEDICINA NUCLEAR Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / ADN Polimerasa beta / Resonancia Magnética Nuclear Biomolecular / Sulfolobus solfataricus Idioma: En Revista: Biomol NMR Assign Asunto de la revista: BIOLOGIA MOLECULAR / MEDICINA NUCLEAR Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos