Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for detection of beet necrotic yellow vein virus.
Arch Virol
; 162(2): 495-500, 2017 Feb.
Article
en En
| MEDLINE
| ID: mdl-27738843
Sugar beet can be infected by many different viruses that can reduce yield; beet necrotic yellow vein virus (BNYVV) is one of the most economically important viruses of this crop plant. This report describes a new reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for identification of BNYVV. In addition, a novel immunocapture (IC) RT-LAMP assay for rapid and easy detection (without RNA extraction) of BNYVV was developed here and compared with DAS-ELISA and RT-LAMP assays. Our results show that the IC-RT-LAMP assay is a highly reliable alternative assay for identification of BNYVV.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus de Plantas
/
Virus ARN
/
Inmunoensayo
/
Técnicas de Amplificación de Ácido Nucleico
/
Beta vulgaris
/
Transcripción Reversa
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Arch Virol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Irán
Pais de publicación:
Austria