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Fabrication and utility of a transparent graphene neural electrode array for electrophysiology, in vivo imaging, and optogenetics.
Park, Dong-Wook; Brodnick, Sarah K; Ness, Jared P; Atry, Farid; Krugner-Higby, Lisa; Sandberg, Amelia; Mikael, Solomon; Richner, Thomas J; Novello, Joseph; Kim, Hyungsoo; Baek, Dong-Hyun; Bong, Jihye; Frye, Seth T; Thongpang, Sanitta; Swanson, Kyle I; Lake, Wendell; Pashaie, Ramin; Williams, Justin C; Ma, Zhenqiang.
Afiliación
  • Park DW; Department of Electrical and Computer Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Brodnick SK; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Ness JP; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Atry F; Department of Electrical Engineering and Computer Science, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
  • Krugner-Higby L; Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Sandberg A; Department of Neuromodulation, Medtronic, Fridley, Minnesota, USA.
  • Mikael S; Department of Electrical and Computer Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Richner TJ; Department of Physiology and Biophysics, University of Washington, Seattle, Washington, USA.
  • Novello J; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Kim H; Department of Electrical and Computer Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Baek DH; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Bong J; Department of Electrical and Computer Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Frye ST; Department of Electrical Engineering and Computer Science, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
  • Thongpang S; Department of Biomedical Engineering, Mahidol University, Bangkok, Thailand.
  • Swanson KI; Department of Neurosurgery, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Lake W; Department of Neurosurgery, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Pashaie R; Department of Electrical Engineering and Computer Science, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
  • Williams JC; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Ma Z; Department of Electrical and Computer Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Nat Protoc ; 11(11): 2201-2222, 2016 Nov.
Article en En | MEDLINE | ID: mdl-27735935
Transparent graphene-based neural electrode arrays provide unique opportunities for simultaneous investigation of electrophysiology, various neural imaging modalities, and optogenetics. Graphene electrodes have previously demonstrated greater broad-wavelength transmittance (∼90%) than other transparent materials such as indium tin oxide (∼80%) and ultrathin metals (∼60%). This protocol describes how to fabricate and implant a graphene-based microelectrocorticography (µECoG) electrode array and subsequently use this alongside electrophysiology, fluorescence microscopy, optical coherence tomography (OCT), and optogenetics. Further applications, such as transparent penetrating electrode arrays, multi-electrode electroretinography, and electromyography, are also viable with this technology. The procedures described herein, from the material characterization methods to the optogenetic experiments, can be completed within 3-4 weeks by an experienced graduate student. These protocols should help to expand the boundaries of neurophysiological experimentation, enabling analytical methods that were previously unachievable using opaque metal-based electrode arrays.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electrodos Implantados / Electrofisiología / Imagen Molecular / Optogenética / Grafito Límite: Animals Idioma: En Revista: Nat Protoc Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electrodos Implantados / Electrofisiología / Imagen Molecular / Optogenética / Grafito Límite: Animals Idioma: En Revista: Nat Protoc Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido