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A bioorthogonal approach for imaging the binding between Dasatinib and its target proteins inside living cells.
Kim, Young-Rang; Kim, Young Hye; Kim, Sung Woo; Lee, Yong Ju; Chae, Dong-Eon; Kim, Kyung-A; Lee, Zee-Won; Kim, Nam Doo; Choi, Jong-Soon; Choi, Insung S; Lee, Kyung-Bok.
Afiliación
  • Kim YR; Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Daejeon 34133, Korea. kblee@kbsi.re.kr.
  • Kim YH; Biomedical Omics Group, Korea Basic Science Institute (KBSI), Cheongju 28119, Korea.
  • Kim SW; Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Daejeon 34133, Korea. kblee@kbsi.re.kr.
  • Lee YJ; Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Daejeon 34133, Korea. kblee@kbsi.re.kr.
  • Chae DE; Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Daejeon 34133, Korea. kblee@kbsi.re.kr and Graduate School of Analytical Science and Technology (GRAST), Chungnam National University, Daejeon 34134, Korea.
  • Kim KA; Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Daejeon 34133, Korea. kblee@kbsi.re.kr and Graduate School of Analytical Science and Technology (GRAST), Chungnam National University, Daejeon 34134, Korea.
  • Lee ZW; Drug Discovery System & Pharmaceuticals, Inc. (DDSPharm), Daejeon 34165, Korea.
  • Kim ND; New Drug Development Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, Korea.
  • Choi JS; Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Daejeon 34133, Korea. kblee@kbsi.re.kr and Graduate School of Analytical Science and Technology (GRAST), Chungnam National University, Daejeon 34134, Korea.
  • Choi IS; Center for Cell-Encapsulation Research and Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea. ischoi@kaist.ac.kr.
  • Lee KB; Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Daejeon 34133, Korea. kblee@kbsi.re.kr.
Chem Commun (Camb) ; 52(79): 11764-11767, 2016 Sep 27.
Article en En | MEDLINE | ID: mdl-27711355
Herein, we present a simple readout of the binding between a chemical drug and its target proteins in the cytoplasm by using a two-step bioorthogonal labeling method combined with spatially-localized expression of proteins. Dasatinib was modified with trans-cyclooctene (TCO), and its cytoplasmic target kinases were expressed in intracellular compartments, such as endosomes and F-actins. After bioorthogonal labeling, the colocalization between Dasatinib and its target proteins was observed in intracellular compartments.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Quinasas / Actinas / Dasatinib / Microscopía Fluorescente Límite: Humans Idioma: En Revista: Chem Commun (Camb) Asunto de la revista: QUIMICA Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Quinasas / Actinas / Dasatinib / Microscopía Fluorescente Límite: Humans Idioma: En Revista: Chem Commun (Camb) Asunto de la revista: QUIMICA Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido