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The scaffold protein Tks4 is required for the differentiation of mesenchymal stromal cells (MSCs) into adipogenic and osteogenic lineages.
Dülk, Metta; Kudlik, Gyöngyi; Fekete, Anna; Ernszt, Dávid; Kvell, Krisztián; Pongrácz, Judit E; Mero, Balázs L; Szeder, Bálint; Radnai, László; Geiszt, Miklós; Csécsy, Dalma E; Kovács, Tamás; Uher, Ferenc; Lányi, Árpád; Vas, Virag; Buday, László.
Afiliación
  • Dülk M; Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
  • Kudlik G; Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
  • Fekete A; Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
  • Ernszt D; Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pecs, Hungary.
  • Kvell K; Szentagothai Research Center, University of Pecs, Hungary.
  • Pongrácz JE; Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pecs, Hungary.
  • Mero BL; Szentagothai Research Center, University of Pecs, Hungary.
  • Szeder B; Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pecs, Hungary.
  • Radnai L; Szentagothai Research Center, University of Pecs, Hungary.
  • Geiszt M; Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
  • Csécsy DE; Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
  • Kovács T; Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
  • Uher F; Department of Physiology, Semmelweis University Medical School, Budapest, Hungary.
  • Lányi Á; "Momentum" Peroxidase Enzyme Research Group of the Semmelweis University and the Hungarian Academy of Sciences, Budapest, Hungary.
  • Vas V; Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
  • Buday L; Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
Sci Rep ; 6: 34280, 2016 10 06.
Article en En | MEDLINE | ID: mdl-27711054
The commitment steps of mesenchymal stromal cells (MSCs) to adipogenic and other lineages have been widely studied but not fully understood. Therefore, it is critical to understand which molecules contribute to the conversion of stem cells into differentiated cells. The scaffold protein Tks4 plays a role in podosome formation, EGFR signaling and ROS production. Dysfunction of Tks4 causes a hereditary disease called Frank-ter Haar syndrome with a variety of defects concerning certain mesenchymal tissues (bone, fat and cartilage) throughout embryogenic and postnatal development. In this study, we aimed to analyze how the mutation of Tks4 affects the differentiation potential of multipotent bone marrow MSCs (BM-MSCs). We generated a Tks4 knock-out mouse strain on C57Bl/6 background, and characterized BM-MSCs isolated from wild type and Tks4-/- mice to evaluate their differentiation. Tks4-/- BM-MSCs had reduced ability to differentiate into osteogenic and adipogenic lineages compared to wild type. Studying the expression profile of a panel of lipid-regulated genes during adipogenic induction revealed that the expression of adipogenic transcription factors, genes responsible for lipid droplet formation, sterol and fatty acid metabolism was delayed or reduced in Tks4-/- BM-MSCs. Taken together, these results establish a novel function for Tks4 in the regulation of MSC differentiation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Fosfoproteínas / Transducción de Señal / Diferenciación Celular / Adipogénesis / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Fosfoproteínas / Transducción de Señal / Diferenciación Celular / Adipogénesis / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Reino Unido