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Identification and Characterization of a Chloroplast-Targeted Obg GTPase in Dendrobium officinale.
Chen, Ji; Deng, Feng; Deng, Mengsheng; Han, Jincheng; Chen, Jianbin; Wang, Li; Yan, Shen; Tong, Kai; Liu, Fan; Tian, Mengliang.
Afiliación
  • Chen J; 1 Agronomy College, Sichuan Agricultural University , Chengdu, China .
  • Deng F; 1 Agronomy College, Sichuan Agricultural University , Chengdu, China .
  • Deng M; 1 Agronomy College, Sichuan Agricultural University , Chengdu, China .
  • Han J; 1 Agronomy College, Sichuan Agricultural University , Chengdu, China .
  • Chen J; 1 Agronomy College, Sichuan Agricultural University , Chengdu, China .
  • Wang L; 1 Agronomy College, Sichuan Agricultural University , Chengdu, China .
  • Yan S; 1 Agronomy College, Sichuan Agricultural University , Chengdu, China .
  • Tong K; 1 Agronomy College, Sichuan Agricultural University , Chengdu, China .
  • Liu F; 1 Agronomy College, Sichuan Agricultural University , Chengdu, China .
  • Tian M; 2 Institute for New Rural Development, Sichuan Agricultural University , Yaan, China .
DNA Cell Biol ; 35(12): 802-811, 2016 Dec.
Article en En | MEDLINE | ID: mdl-27710025
Bacterial homologous chloroplast-targeted Obg GTPases (ObgCs) belong to the plant-typical Obg group, which is involved in diverse physiological processes during chloroplast development. However, the evolutionarily conserved function of ObgC in plants remains elusive and requires further investigation. In this study, we identified DoObgC from an epiphytic plant Dendrobium officinale and demonstrated the characteristics of DoObgC. Sequence analysis indicated that DoObgC is highly conserved with other plant ObgCs, which contain the chloroplast transit peptide (cTP), Obg fold, G domain, and OCT regions. The C terminus of DoObgC lacking the chloroplast-targeting cTP region, DoObgCΔ1-160, showed strong similarity to ObgE and other bacterial Obgs. Overexpression of DoObgCΔ1-160 in Escherichia coli caused slow cell growth and an increased number of elongated cells. This phenotype was consistent with the phenotype of cells overexpressing ObgE. Furthermore, the expression of recombinant DoObgCΔ1-160 enhanced the cell persistence of E. coli to streptomycin. Results of transient expression assays revealed that DoObgC was localized to chloroplasts. Moreover, we demonstrated that DoObgC could rescue the embryotic lethal phenotype of the Arabidopsis obgc-t mutant, suggesting that DoObgC is a functional homolog to Arabidopsis AtObgC in D. officinale. Gene expression profiles showed that DoObgC was expressed in leaf-specific and light-dependent patterns and that DoObgC responded to wounding treatments. Our previous and present studies reveal that ObgC has an evolutionarily conserved role in ribosome biogenesis to adapt chloroplast development to the environment.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Señales de Clasificación de Proteína / Cloroplastos / Regulación de la Expresión Génica de las Plantas / Dendrobium / Proteínas de Cloroplastos / GTP Fosfohidrolasas Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: DNA Cell Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Buscar en Google
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Señales de Clasificación de Proteína / Cloroplastos / Regulación de la Expresión Génica de las Plantas / Dendrobium / Proteínas de Cloroplastos / GTP Fosfohidrolasas Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: DNA Cell Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos