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Dynamics of Base Excision Repair at the Maternal-Fetal Interface in Pregnancies Complicated by Preeclampsia.
Tadesse, Serkalem; Norwitz, Nicholas G; Guller, Seth; Arcuri, Felice; Toti, Paolo; Norwitz, Errol R; Kidane, Dawit.
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  • Tadesse S; 1 Department of Obstetrics and Gynecology, Tufts University School of Medicine, Boston, MA, USA.
  • Norwitz NG; 2 Mother Infant Research Institute, Tufts Medical Center, Boston, MA, USA.
  • Guller S; 3 Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.
  • Arcuri F; 3 Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.
  • Toti P; 4 Department of Molecular and Developmental Medicine, University of Siena, Siena, Italy.
  • Norwitz ER; 5 Department of Medical Biotechnologies, University of Siena, Siena, Italy.
  • Kidane D; 1 Department of Obstetrics and Gynecology, Tufts University School of Medicine, Boston, MA, USA.
Reprod Sci ; 24(6): 856-864, 2017 06.
Article en En | MEDLINE | ID: mdl-27707956
Preeclampsia (PE) (gestational proteinuric hypertension) is the leading cause of maternal and perinatal mortality worldwide. Although placental endothelial dysfunction and oxidative stress are known to contribute to PE, the exact pathological basis for this disorder remains unclear. Previously, we demonstrated that DNA damage at the maternal-fetal interface is more common in the placentas of women with PE than normotensive controls. In this study, we utilized an in vivo comparative study, including 20 preeclamptic women and 8 healthy control subjects, and an in vitro hypoxia/reperfusion model to mimic the effects of oxidative stress at the maternal-fetal interface. We tracked the spatial pattern of expression of 2 base excision repair proteins, 8-oxoguanine glycosylase (OGG1) and apurinic/apyrimidinic endonuclease-1 (APE1), at the maternal-fetal interface in response to oxidative stress. In vivo, we found a significant increase in OGG1 and APE1 concentrations in PE placental tissues as compared to normotensive controls ( P < .0001). Further, our in vitro study revealed that OGG1 and APE1 expression is much greater in maternal cells (decidua) than in fetal cells (cytotrophoblasts) of placental tissue subjected to oxidative stress ( P < .0001). Our results suggest that OGG1 and APE1 likely protect decidual cells from oxidative base damage.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Preeclampsia / Estrés Oxidativo / Reparación del ADN Límite: Female / Humans / Pregnancy Idioma: En Revista: Reprod Sci Asunto de la revista: MEDICINA REPRODUTIVA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Preeclampsia / Estrés Oxidativo / Reparación del ADN Límite: Female / Humans / Pregnancy Idioma: En Revista: Reprod Sci Asunto de la revista: MEDICINA REPRODUTIVA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos