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Identification, Molecular Cloning of IL-1ß and Its Expression Profile during Nocardia seriolae Infection in Largemouth Bass, Micropterus salmoides.
Ho, Ping-Yueh; Byadgi, Omkar; Wang, Pei-Chyi; Tsai, Ming-An; Liaw, Li-Ling; Chen, Shih-Chu.
Afiliación
  • Ho PY; Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. pyho@mdu.edu.tw.
  • Byadgi O; Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. omkarcof1@gmail.com.
  • Wang PC; Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. pc921003@gmail.com.
  • Tsai MA; Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. william878588@gmail.com.
  • Liaw LL; Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan. lll@firdi.org.tw.
  • Chen SC; Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. scchen@mail.npust.edu.tw.
Int J Mol Sci ; 17(10)2016 Oct 01.
Article en En | MEDLINE | ID: mdl-27706080
In the present study, IL-1ß cDNA was identified and analyzed from largemouth bass (Micropterus salmoides). Full length IL-1ß mRNA was obtained using Rapid Amplification of cDNA Ends (RACE), which contains 78 bp 3'-UTR, a 455 bp 5'-UTR, and an open reading frame (ORF) of 702 bp coding for 233 amino acid residues. The molecular weight and theoretical isoelectric point of largemouth bass IL-1ß protein was predicted to be 26.7 kDa and 6.08 respectively. A largemouth bass IL-1ß phylogenetic analysis showed a close relation to the IL-1ßs of striped trumpeter (Latris lineata), Chinese perch (Siniperca chuatsi), and Japanese sea bass (Lateolabrax japonicus). Peptidoglycan upregulated IL-1ß in the spleen and head kidney, while lipopolysaccharide upregulated detectable levels of IL-1ß in the spleen only. Largemouth bass, challenged with Nocardia seriolae (1.0 × 106 cfu/mL), showed a significant increase in IL-1ß at 3 and 5 days post infection (dpi) in the spleen, while in the head kidney significant expression was found at 2 and 3 dpi, peaking at 3 dpi. Furthermore, tumor necrosis factor α (TNF-α) showed significantly higher expression in the spleen at 3 and 5 dpi, and in the head kidney at 1 and 3 dpi, with expression decreasing at 5 dpi in both tissues.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lubina / Regulación de la Expresión Génica / Interleucina-1beta / Enfermedades de los Peces / Nocardiosis Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Int J Mol Sci Año: 2016 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lubina / Regulación de la Expresión Génica / Interleucina-1beta / Enfermedades de los Peces / Nocardiosis Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Int J Mol Sci Año: 2016 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza