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Spontaneous Ca2+ transients in mouse microglia.
Korvers, Laura; de Andrade Costa, Amanda; Mersch, Martin; Matyash, Vitali; Kettenmann, Helmut; Semtner, Marcus.
Afiliación
  • Korvers L; Max-Delbrueck-Centrum for Molecular Medicine (MDC) in the Helmholtz Association, Cellular Neurosciences, Robert-Roessle-Str. 10, 13092 Berlin, Germany.
  • de Andrade Costa A; Max-Delbrueck-Centrum for Molecular Medicine (MDC) in the Helmholtz Association, Cellular Neurosciences, Robert-Roessle-Str. 10, 13092 Berlin, Germany.
  • Mersch M; Max-Delbrueck-Centrum for Molecular Medicine (MDC) in the Helmholtz Association, Cellular Neurosciences, Robert-Roessle-Str. 10, 13092 Berlin, Germany.
  • Matyash V; Max-Delbrueck-Centrum for Molecular Medicine (MDC) in the Helmholtz Association, Cellular Neurosciences, Robert-Roessle-Str. 10, 13092 Berlin, Germany.
  • Kettenmann H; Max-Delbrueck-Centrum for Molecular Medicine (MDC) in the Helmholtz Association, Cellular Neurosciences, Robert-Roessle-Str. 10, 13092 Berlin, Germany.
  • Semtner M; Max-Delbrueck-Centrum for Molecular Medicine (MDC) in the Helmholtz Association, Cellular Neurosciences, Robert-Roessle-Str. 10, 13092 Berlin, Germany. Electronic address: marcus.semtner@mdc-berlin.de.
Cell Calcium ; 60(6): 396-406, 2016 12.
Article en En | MEDLINE | ID: mdl-27697289
Microglia are the resident immune cells in the central nervous system and many of their physiological functions are known to be linked to intracellular calcium (Ca2+) signaling. Here we show that isolated and purified mouse microglia-either freshly or cultured-display spontaneous and transient Ca2+ elevations lasting for around ten to twenty seconds and occurring at frequencies of around five to ten events per hour and cell. The events were absent after depletion of internal Ca2+ stores, by phospholipase C (PLC) inhibition or blockade of inositol-1,4,5-trisphosphate receptors (IP3Rs), but not by removal of extracellular Ca2+, indicating that Ca2+ is released from endoplasmic reticulum intracellular stores. We furthermore provide evidence that autocrine ATP release and subsequent activation of purinergic P2Y receptors is not the trigger for these events. Spontaneous Ca2+ transients did also occur after stimulation with Lipopolysaccharide (LPS) and in glioma-associated microglia, but their kinetics differed from control conditions. We hypothesize that spontaneous Ca2+ transients reflect aspects of cellular homeostasis that are linked to regular and patho-physiological functions of microglia.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Microglía Límite: Animals Idioma: En Revista: Cell Calcium Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Microglía Límite: Animals Idioma: En Revista: Cell Calcium Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Países Bajos