Cleavage of influenza RNA by using a human PUF-based artificial RNA-binding protein-staphylococcal nuclease hybrid.

Mori, Tomoaki; Nakamura, Kento; Masaoka, Keisuke; Fujita, Yusuke; Morisada, Ryosuke; Mori, Koichi; Tobimatsu, Takamasa; Sera, Takashi

Mori, Tomoaki; Nakamura, Kento; Masaoka, Keisuke; Fujita, Yusuke; Morisada, Ryosuke; Mori, Koichi; Tobimatsu, Takamasa; Sera, Takashi.

Afiliación

Mori T; Department of Applied Chemistry and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Kita-ku, Okayama 700-8530, Japan.
Nakamura K; Department of Applied Chemistry and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Kita-ku, Okayama 700-8530, Japan.
Masaoka K; Department of Applied Chemistry and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Kita-ku, Okayama 700-8530, Japan.
Fujita Y; Department of Applied Chemistry and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Kita-ku, Okayama 700-8530, Japan.
Morisada R; Department of Applied Chemistry and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Kita-ku, Okayama 700-8530, Japan.
Mori K; Department of Applied Chemistry and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Kita-ku, Okayama 700-8530, Japan.
Tobimatsu T; Department of Applied Chemistry and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Kita-ku, Okayama 700-8530, Japan.
Sera T; Department of Applied Chemistry and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Kita-ku, Okayama 700-8530, Japan. Electronic address: sera@cc.okayama-u.ac.jp.

Biochem Biophys Res Commun ; 479(4): 736-740, 2016 Oct 28.

Article en En | MEDLINE | ID: mdl-27693585

Asunto(s)

Subtipo H1N1 del Virus de la Influenza A/metabolismo; Nucleasa Microcócica/metabolismo; ARN Viral/metabolismo; Proteínas de Unión al ARN/metabolismo; Animales; Estudios de Factibilidad; Humanos; Subtipo H1N1 del Virus de la Influenza A/genética; Cinética; Nucleasa Microcócica/genética; ARN Viral/genética; Proteínas de Unión al ARN/genética; Proteínas Recombinantes de Fusión/genética; Proteínas Recombinantes de Fusión/metabolismo; Especificidad por Sustrato; Inactivación de Virus

Palabras clave

Artificial RNA restriction enzyme; RNA cleavage; RNA virus

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Viral / Proteínas de Unión al ARN / Subtipo H1N1 del Virus de la Influenza A / Nucleasa Microcócica Límite: Animals / Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2016 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos

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