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T-cell factor-4 and MHC upregulation in pigs receiving a live attenuated classical swine fever virus (CSFV) vaccine strain with interferon-gamma adjuvant.
Fan, Y-H; Lin, Y-L; Hwang, Y-C; Yang, H-C; Chiu, H-C; Chiou, S-H; Jong, M-H; Chow, K-C; Lin, C-C.
Afiliación
  • Fan YH; Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 40227, Taiwan, Republic of China.
  • Lin YL; Epidemiology Research Division, Animal Health Research Institute, Council of Agriculture, Executive Yuan, Tamsui, New Taipei City 251, Taiwan, Republic of China.
  • Hwang YC; Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 40227, Taiwan, Republic of China.
  • Yang HC; Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 40227, Taiwan, Republic of China.
  • Chiu HC; Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 40227, Taiwan, Republic of China.
  • Chiou SH; Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 40227, Taiwan, Republic of China. Electronic address: shchiou@dragon.nchu.edu.tw.
  • Jong MH; Hog Cholera Division, Animal Health Research Institute, Council of Agriculture, Executive Yuan, Tamsui, New Taipei City 251, Taiwan, Republic of China.
  • Chow KC; Institute of Biomedical Sciences, National Chung Hsing University, Taichung 40227, Taiwan, Republic of China.
  • Lin CC; Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan, Republic of China.
Vet J ; 216: 148-56, 2016 Oct.
Article en En | MEDLINE | ID: mdl-27687943
The effect of co-administration of interferon (IFN)-γ in pigs undergoing vaccination with an attenuated strain (LPC) of classical swine fever virus (CSFV) was investigated. Unvaccinated pigs demonstrated pyrexia and died 7-9 days after challenge with virulent CSFV. Pigs receiving the attenuated vaccine remained healthy after virus challenge, except for mild, transient pyrexia, whereas pigs receiving IFN-γ simultaneously with the vaccine demonstrated normal body temperatures after virus challenge. Examination by nested RT-PCR revealed greater viral load in the spleens of the pigs vaccinated with the attenuated CSFV, compared with those that had additionally received IFN-γ. Expression of major histocompatibility complex (MHC) class I and MHC class II molecules was upregulated in the spleens of the IFN-γ treated vaccinated pigs, demonstrated by immunohistochemistry. Based on Western blot analysis, anti-CSFV IgG2 antibodies were elevated in vaccinated pigs by co-administration of IFN-γ (IFN-γ(Hi): P < 0.01; IFN-γ(Lo): P <0.05). By employing the suppression subtractive hybridization technique, RT-PCR, in situ hybridization, and immunohistochemistry, T-cell factor-4 (Tcf-4) mRNA and protein expression were found to be upregulated in the spleens of vaccinated pigs that had received IFN-γ. This study suggests involvement of Tcf-4 in IFN-γ-mediated immune regulation following CSFV vaccination.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas Virales / Peste Porcina Clásica / Virus de la Fiebre Porcina Clásica Límite: Animals Idioma: En Revista: Vet J Asunto de la revista: MEDICINA VETERINARIA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas Virales / Peste Porcina Clásica / Virus de la Fiebre Porcina Clásica Límite: Animals Idioma: En Revista: Vet J Asunto de la revista: MEDICINA VETERINARIA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido