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Recombinant scFv antibodies against infectious pancreatic necrosis virus isolated by flow cytometry.
Xu, Li-Ming; Zhao, Jing-Zhuang; Liu, Miao; Cao, Yong-Sheng; Yin, Jia-Sheng; Liu, Hong-Bai; Lu, Tongyan.
Afiliación
  • Xu LM; Heilongjiang River Fishery Research Institute Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: 279463437@qq.com.
  • Zhao JZ; Heilongjiang River Fishery Research Institute Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: 422842454@qq.com.
  • Liu M; Heilongjiang River Fishery Research Institute Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: 446048847@qq.com.
  • Cao YS; Heilongjiang River Fishery Research Institute Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: 375825652@qq.com.
  • Yin JS; Heilongjiang River Fishery Research Institute Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: xwsc20@tom.com.
  • Liu HB; Heilongjiang River Fishery Research Institute Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: 112893129@qq.com.
  • Lu T; Heilongjiang River Fishery Research Institute Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: lutongyan@hotmail.com.
J Virol Methods ; 237: 204-209, 2016 11.
Article en En | MEDLINE | ID: mdl-27678027
Infectious pancreatic necrosis is a significant disease of farmed salmonids in China. In this study, a single chain variable fragment (scFv) antibody library derived from rainbow trout (Oncorhynchus mykiss) and viral protein VP2 of a Chinese infectious pancreatic necrosis virus (IPNV) isolate ChRtm213 were co-expressed by a bacterial display technology. The library was subjected to three rounds of screening by flow cytometry (FCM) to select IPNV specific antibodies. Six antibody clones with different mean fluorescence intensities (MFI) were obtained by picking colonies at random. The antibody clones were expressed and purified. The purified IPNV-specific scFv antibodies were used successfully in Western blotting, enzyme linked immunosorbent assay (ELISA) and an immunofluorescence antibody test (IFAT). This method provides a high throughput means to screen an antibody library by flow cytometry, and isolate a panel of antibody that can be used as potential reagents for the detection and study of IPNV that are prevalent in China.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Inmunológicas / Virus de la Necrosis Pancreática Infecciosa / Infecciones por Birnaviridae / Anticuerpos de Cadena Única / Citometría de Flujo / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: J Virol Methods Año: 2016 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Inmunológicas / Virus de la Necrosis Pancreática Infecciosa / Infecciones por Birnaviridae / Anticuerpos de Cadena Única / Citometría de Flujo / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: J Virol Methods Año: 2016 Tipo del documento: Article Pais de publicación: Países Bajos